You Searched For: Enzo Life Sciences

Catalog Number: (10136-002)

Supplier: Enzo Life Sciences

Description: Fastest method for complete, high-throughput (96-well) bisulfite conversion of DNA for methylation analysis.

SDS

Catalog Number: (10136-010)

Supplier: Enzo Life Sciences

Description: Enrich for methylated DNA (100-fold) from sample for complete diverse downstream applications

SDS

Catalog Number: (10050-248)

Supplier: Enzo Life Sciences

Description: VEGF (Vascular endothelial growth factor) is a potent polypeptide regulator of blood vessel function. VEGF promotes an array of responses in the endothelium, including hyperpermeability, endothelial cell growth, angiogenesis, and enhanced glucose transport. Several alternate splice isoforms of VEGF are known. Two high-affinity receptors for VEGF, VEGFR1 and VEGFR2, have been identified and characterized.

Catalog Number: (10050-132)

Supplier: Enzo Life Sciences

Description: Nucleolin (identical to human DNA helicase IV) is an abundant, non-ribosomal protein of the nucleolus involved in the organization of nuclear chromatin, rDNA transcription, packaging of pre-RNA, ribosome assembly, nucleocytoplasmic transport, cytokinesis, nucleogenesis, and apoptosis. A predominantly nuclear protein, the presence of nucleolin at the surface of cancer cells suggests that it could be valuable as a marker for the diagnosis of cancer.

Catalog Number: (10050-184)

Supplier: Enzo Life Sciences

Description: Hsp20 is a member of the family of small heat shock proteins (sHsps), with homology to Hsp25, Hsp27, and alphaB-crystallin. Highest expression of Hsp20 is found in skeletal, smooth, and cardiac muscle. Under heat shock, Hsp20 translocates from the cytosol to the nucleus, although the amount of Hsp20 in the cell does not increase like other Hsps. Hsp20 oligomerization and interaction with polymeric actin filaments is regulated via phosphorylation by PKA and PKG.

Catalog Number: (10050-212)

Supplier: Enzo Life Sciences

Description: The deubiquitinylating enzyme USP19 is induced in skeletal muscle atrophy in response to numerous catabolic stimuli, including cancer, fasting, diabetes, and glucocorticoids. Loss of USP19 interferes with the growth of L6 myoblasts through its modulation of the transcription of major myofibrillar proteins.

USP19 regulates cell growth, partly through indirect control of p27Kip1 degradation in the G1 phase of the cell cycle. Since a number of cancers are associated with enhanced degradation of p27Kip1, the overexpression of USP19 may also be a pathogenetic mechanism of the disordered cell growth in cancer and a potential novel therapeutic target for its treatment.

In addition USP19 has been shown to be a tail anchored DUB, localised to the ER, suggesting a role in the turnover of ERAD substrates and to be targeted by the unfolded protein response.

USP19 is expressed in a wide range of tissues and is localised to the cytoplasm and endoplasmic reticulum.

Catalog Number: (10050-208)

Supplier: Enzo Life Sciences

Description: When cells are stimulated with pro-inflammatory cytokines, most of their constitutively expressed proteasomes are replaced with immunoproteasomes, which increase the production of peptides for presentation on MHC class I molecules. In addition, cortical thymic epithelial cells selectively express a type of proteasome known as the thymoproteasome, in which the β5i immunoproteasome subunit has been replaced by the thymus specific proteasome subunit β5t. The thymoproteasome is required for the positive selection of thymocytes and shaping the T cell receptor repertoire of cytotoxic T lymphocytes. In addition mice deficient in β5t display defective development of CD8(+) T cells in the thymus suggesting a key role for β5t and the thymoproteasome in generating MHC class I-restricted CD8(+) T cells.

Catalog Number: (10051-924)

Supplier: Enzo Life Sciences

Description: The Src homology 2 domain containing (Shc) adaptor protein links growth factor, cytokine, and antigen receptor stimulation to the Ras signaling pathway, and plays a critical role in insulin signaling, tumorigenesis, and hematopoietic cell growth and differentiation. Structurally, Shc is composed of an N-terminal phosphotyrosine binding (PTB) domain, a medial CH1 domain, and a C-terminal SH2 domain. Upon binding to activate cell-surface receptors, Shc is phosphorylated at three tyrosine residues (Tyr239, Tyr240, Tyr317) triggering recognition of Shc by SH2-containing proteins such as Grb2.

Catalog Number: (10051-898)

Supplier: Enzo Life Sciences

Description: The transcription factor CREB (cAMP response element binding) binds the palindromic octanucleotide TGACGTCA. Members of the ATF/CREB family include CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2, and ATF-3. Phosphorylation of CREB allows recruitment of CREB-binding protein (CBP), a large co-activator that contacts the general transcriptional machinery. CREB-mediated transcription regulates diverse cellular responses, including intermediary metabolism, neuronal signaling, cell proliferation, and apoptosis.

Catalog Number: (10051-942)

Supplier: Enzo Life Sciences

Description: Leukotrienes are metabolic products of arachidonic acid formed by the action of 5-lipoxygenase (5-LO), with the principal products being leukotrienes C4 (LTC4) and B4 (LTB4). Leukotriene B4 (LTB4) stimulates leukocyte functions including lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB4 has been implicated as a potent mediator of inflammatory diseases and immunoregulation. The cysteinyl leukotrienes (LTC4, LTD4, and LTE4) are formed by addition of cysteine derivatives to LTA4. The conversion of LTA4 to LTC4 by the enzyme LTC4 synthase limits the rate of cysteinyl leukotriene formation. Cysteinyl leukotrienes are potent lipid mediators in inflammation, inducing smooth muscle contractions and increased capillary permeability.

Catalog Number: (10051-894)

Supplier: Enzo Life Sciences

Description: Bad is an ~22 kDa pro-apoptotic protein that aids in the initiation of cell death. It exhibits sequence homology to Bcl-2, a protein that prevents apoptosis, within its BH1 and BH2 domains. Bad is phosphorylated at several sites including Ser112, Ser136; phosphorylation by kinases like Akt is known to alter the effects of Bad. Bad has been demonstrated to be phosphorylated at Ser170.

Catalog Number: (10051-932)

Supplier: Enzo Life Sciences

Description: The cannabinoid receptors CB1 and CB2 are GPCRs named for their signaling function in response to cannabinoid drugs such as Delta9-tetrahydrocannabinol (Delta9-THC). In addition to Delta9-THC and its synthetic analogs, CB1 and CB2 receptors are bound by endogenous eicosanoid ligands (e.g. anandamide), and signal via Gi/o alpha subunits to regulate adenylyl cyclase activity (generally inhibitory), ion channel activation, intracellular Ca2+ levels, nitric oxide synthase activation, and immediate early gene expression. CB1 and CB2 signals in turn regulate neurological and immunological functions such as inhibition of neurotransmitter release and immune cell migration, antigen processing, and cytokine production.

Catalog Number: (10049-398)

Supplier: Enzo Life Sciences

Description: The GST superfamily of enzymes play an important role in detoxification of xenobiotics by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. There are four main GST classes; alpha, mu, pi, and tau. GST-P is a member of the pi class of GSTs, and its expression is specifically increased in the early stages of rat hepatocarcinogenesis. Due to its high affinity for glutathione, GST is frequently utilized as an approximately 26 kDa fusion tag for the expression and purification of recombinant proteins.

Catalog Number: (10049-490)

Supplier: Enzo Life Sciences

Description: Monoclonal Antibody, Primary Antibody, Used in ICC, IF, IHC, WB with species reactivity to Human, Mouse, Rat, Bovine, Chicken, Host: Mouse, Immunogen: Bovine alphaB-crystallin, Isotype: IgG1

Catalog Number: (10049-482)

Supplier: Enzo Life Sciences

Description: Primary Antibody, Used in IF, IHC, IP, WB with species reactivity to Human, Mouse, Rat, Clam, Host: Rabbit, Immunogen: Native human Cu/Zn SOD

Catalog Number: (10049-462)

Supplier: Enzo Life Sciences

Description: The green fluorescent protein (GFP) is derived from the jellyfish Aequorea victoria, that fluoresces green when exposed to blue light. GFP is a frequently used reporter for gene expression in recombinant DNA and cell transfection systems.