Advancing Adeno-Associated Virus (AAV) Characterization: High-Resolution Techniques for Gene Therapy Applications

The purity and homogeneity of Adeno-Associated Virus (AAV) vectors are critical for the success of treatment delivery in gene therapy. This webinar will delve into cutting-edge methodologies for the precise characterization and quantification of AAV samples, focusing on the use of high-resolution size-exclusion chromatography (SEC) and strong anion exchange chromatography (SAX).

We will explore the application of the Thermo Scientific™ SurePac™ Bio 550 SEC MDi™ column, which leverages 3 µm monodisperse silica particles for superior separation of AAV monomeric capsids from high molecular weight species (HMWS). The column's enhanced resolution and reproducibility significantly improve analytical throughput and sample integrity, making it an excellent choice for both research and industrial settings.

Attendees will gain insights into the practical applications of these advanced chromatographic methods, including the integration of multi-angle light scattering (MALS) detection for comprehensive AAV titer and aggregate quantification. Additionally, this webinar will cover the use of the Thermo Scientific™ ProPac™ 3R SAX chromatography column for the separation of empty and full AAV capsids. This technique offers high-resolution separation and consistent performance, streamlining method development and optimization.

Join us to learn how these innovative approaches can enhance the quality assurance and production efficiency of AAV-based gene therapies.

Key Takeaways:

• High-resolution separation of AAV monomeric capsids and HMWS using SEC
• Integration of UV, fluorescence, and MALS detection for robust AAV characterization
• Effective differentiation of empty and full AAV capsids with SAX
• Practical examples and case studies demonstrating the application of these techniques in biopharmaceutical research and development

For more information, please contact us at webinar@avantorsciences.com

Presented by:

Ashleigh Koontz Global Product Manager for Thermo Scientific Bio LC Columns Ashleigh Koontz is a Global Product Manager for Thermo Scientific Bio LC Columns and has launched innovative products that revolutionized the field of size exclusion chromatography with monodisperse inert (MDi) technology. She holds a Bachelor of Science in Chemistry from Georgia Southern University and a Master of Science in Chemistry from the University of North Carolina at Wilmington. Following her education, Ashleigh started her career as a method development chemist, specializing in liquid chromatography sample analysis. Her expertise in this area allowed her to gain valuable insights and contribute to the advancement of chromatography techniques. In addition to her technical background, Ashleigh had the opportunity to gain valuable sales experience as a chemistry specialist for chromatography (LC & GC), spectroscopy, sample prep, and chemical standards. We are honored to have her speak with us today to keep updated on market dynamics and processes to drive better efficiencies in the lab with updated technology.