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Aequorea victoria Recombinant GST-eGFP Fusion Protein, DYKDDDDK-Tag (from E. coli)

Supplier: CUBE BIOTECH
Description: Enhanced GFP (eGFP) is a modified version of GFP that exhibits even stronger fluorescence for easier detection.

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HiYield-T7 Cell-free E. coli Lysate

Supplier: CUBE BIOTECH
Description: Our <i>E. coli</i> cell-free lysates are the isolated protein purification machinery of bacterial cells. They create a high-yielding and adjustable cell-free protein expression system.

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HiYield Cell-free E. coli Lysate

Supplier: CUBE BIOTECH
Description: Our <i>E. coli</i> cell-free lysates are the isolated protein purification machinery of bacterial cells. They create a high-yielding and adjustable cell-free protein expression system.

Product Image-77392-790

Mouse Recombinant MSP2N2 (from E. coli)

Supplier: CUBE BIOTECH
Description: Membrane scaffold protein, mouse origin.

Product Image-77392-906

PureCube Ni-IDA MagBeads

Supplier: CUBE BIOTECH
Description: PureCube Ni-IDA MagBeads were developed by Cube Biotech for His-tag protein purification.

Product Image-77392-746

Nanodisc MSP2N2-His DMPC

Catalog Number: (77392-746)
Supplier: CUBE BIOTECH
Description: This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.
Product Image-77392-826

Nanodisc Rat MSP1E3D1-His DMPC

Catalog Number: (77392-826)
Supplier: CUBE BIOTECH
Description: Membrane scaffold protein; rat origin.
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AASTY 6-50

Supplier: CUBE BIOTECH
Description: AASTYs (Acrylic acid-co-styrenes) - like AASTY 6-50 - are highly-alternating copolymers, well-suited for the generation of native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 6-50 gets its name from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general lighter AASTYs, like 6-50 tend to be more aggressive, while heavier AASTYs, such as 11-45 show higher thermodynamic stability.

The exact composition of AASTY copolymers shows different extraction efficiency, depending on the lipid composition of the lipid bilayers being formulated into nanodiscs. As AASTY is made using controlled radical polymerization techniques, the dispersity of polymer molecular weight distribution is low, and the molecular weights are controlled. This means that excess AASTY copolymer can be removed by dialysis after nanodisc formation. Based on previous findings on SMA, it is the expectation that AASTY of different molecular weights will display different rates of nanodisc formation, extraction efficacy, and stability of resulting nanodiscs.

Every membrane protein solubilization needs to undergo a screening process before. The characteristic phospholipid environment surrounding the different membrane proteins in question performs differently well with each polymer. To support you in this process we offer a handy Screening Kit for AASTYs to test them all. Additionally, we recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022

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AASTY 11-50

Supplier: CUBE BIOTECH
Description: AASTYs (Acrylic acid-co-styrenes) - like AASTY 11-50 - are highly-alternating copolymers, well-suited for the generation of native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 11-50 gets its name from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general lighter AASTYs, like 6-45 tend to be more aggressive, while heavier AASTYs, such as 11-50 show higher thermodynamic stability.

Every membrane protein solubilization needs to undergo a screening process in before. The characteristic phospholipid environment surrounding the different membrane proteins in question performs differently well with each polymer. To support you in this process, we offer a handy Screening Kit for AASTYs to test them all.

We recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022

Product Image-77392-464

FPLC Column/Cartridge Assembly Press

Supplier: CUBE BIOTECH
Description: The FPLC Cartridge assembly press is an efficient tool that simplifies the packaging process for FPLC columns.

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AASTY 11-45

Supplier: CUBE BIOTECH
Description: AASTYs (Acrylic acid-co-styrenes) - like AASTY 11-45 - are highly alternating copolymers, well-suited for the generation of native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 11-45 gets its name from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general lighter AASTYs, like 6-45 tend to be more aggressive, while heavier AASTYs, such as 11-45 show higher thermodynamic stability.

The exact composition of AASTY copolymers shows different extraction efficiency, depending on the lipid composition of the lipid bilayers being formulated into nanodiscs. As AASTY is made by controlled radical polymerization techniques, the dispersity of polymer molecular weight distribution is low, and the molecular weights are controlled. This means that excess AASTY copolymer can be removed by dialysis after nanodisc formation. Based on previous findings on SMA, it is the expectation that AASTY of different molecular weights will display different rates of nanodisc formation, extraction efficacy, and stability of resulting nanodiscs.

Every membrane protein solubilization needs to undergo a screening process before. The characteristic phospholipid environment surrounding the different membrane proteins in question performs differently well with each polymer. To support you in this process, we offer a handy Screening Kit for AASTYs to test them all. Additionally, we recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022

Product Image-77392-852

SARS-CoV-2 Recombinant SARS CoV-2 Spike Protein in LMNG Detergent (from HEK293 cells)

Supplier: CUBE BIOTECH
Description: The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor.

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SMALP 140-I

Supplier: CUBE BIOTECH
Description: The SMALP products have been developed for biomedical purposes, in particular, for the solubilization of (biological) lipid membranes and isolation & purification of integral membrane proteins.
After solving the SMA + buffer powder in water, the product is ready-to-use. It can primarily used for all cell membrane hosts, including bacteria, yeast, and human cells. After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.

Product Image-77392-764

Nanodisc MSP1D1 dH5-His POPC Biotinyl PE

Catalog Number: (77392-764)
Supplier: CUBE BIOTECH
Description: This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.
Product Image-77392-690

Nanodisc Assembly Kit MSP1D1 dH5-His DMPC

Catalog Number: (77392-690)
Supplier: CUBE BIOTECH
Description: This product is a MSP nanodisc assembly kit. It contains a vial of lyophilized membrane scaffold protein MSP1D1 dh5 (His-tagged) and a vial of DMPC or POPC phopshospholipid to assemble a MSP nanodisc.
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MAGBEADS POLYHUNTER 25ML

Catalog Number: (77392-640)
Supplier: CUBE BIOTECH
Description: MAGBEADS POLYHUNTER 25ML
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
To process your orders without delay, please provide the required business documentation to purchase this product.

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* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.

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This product has been blocked by your organization. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at 1-800-932-5000.
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