Empty 1-step batch columns are ideally suited for batch purifications. They can be filled with your preferred affinity resin, allowing binding, washing, and elution in one spin column.
- Usage:Specific binding and purification of proteins
- Specificity: Depending on the affinity of the used agarose resin
- Binding capacity: Also depending on the used agarose
- Body material: Polypropylene
- Filter pore size: 0.1 to 0.2 µm low binding PVDF
- Max. volume: 600 µl (mini)
- Max. g force: 12000 to 14000×g (45° fixed angle rotor)
- Min. g force: 2500×g for 1 min
Specifically designed for small to mid-scale protein purifications, PureCube 1-step batch spin columns save time and pipetting steps. Containing a SelfSeal membrane technology, the column retains resin and sample in a chamber for batch incubation. By centrifugation, the membrane pores dilate, and the filtered eluate gathers in the collection chamber of the column. PureCube 1-step batch spin columns are available in two sizes: Mini for expression trials, small-scale screening, and other small-volume purification needs or Midi Plus for up to 20 ml volumes.
Required equipment:
-Lysis buffer
-Wash buffer
-Elution buffer
-Ice bath
-Microcentrifuge with rotor suitable for 2 ml centrifuge tubes (min 10000×g)
-2 ml centrifuge tubes
-Micropipettor and Micropipetting tips
-Disposable gravity flow columns with capped bottom outlet, 2 ml
-pH meter
-End-over-end shaker
-SDS-PAGE buffers, reagents and equipment; Optional: Western Blot reagents and equipment
