You Searched For: MP BIOMEDICALS (FKA ICN BIOMED

Catalog Number: (75856-402)

Supplier: MP Biomedicals

Description: Cell lysis solution used for the isolation of genomic DNA from yeast, algae and fungi.

SDS

Supplier: MP Biomedicals

Description: Chloroform is typically used as a solvent and as a cleansing agent. Procedures have been described for the use of chloroform in lambda plaques storage, lambda cDNA storage, the removal of mineral oil from PCR reaction samples, oligonucleotide purification, a hydroxyl radical footprinting protocol, a transcriptional run-on assay protocol, and an overlay assay for beta-galactosidase activity. It has also been used with phenol in such procedures as DNA recovery from polyacrylamide gels, ethidium bromide removal from DNA preparations, lysis protocols for plasmid DNA isolation, RNase removal, and purification of yeast DNA. A protocol describes the use of chloroform in a high-performance thin-layer chromatography protocol for sphingomyelin analysis. Chloroform can also be used in chloroform/methanol mixtures for the isolation of cardiolipids from Geobacillus stearothermophilus and their subsequent MS analysis. The isolation of the bacteriocin amylovorin L471 from Lactobacillus amylovorus DCE 471 in culture broth has been reported, using chloroform/methanol extraction and precipitation in the procedure. Chloroform/2-butanol mixtures can be used for the extraction of steroid sulfates for analysis by nanoelectrospray ionization mass spectrometry.

Supplier: MP Biomedicals

Description: Catalase is used for the removal of peroxides, the generation of oxygen.

Supplier: MP Biomedicals

Description: Cytidine is a pyrimidine nucleoside that is composed of the base cytosine linked to the five-carbon sugar d-ribose. There are a variety of cytidine analogs with potentially useful pharmacology .
Cytidine when intraperitoneal administered in combination with choline improves nerve regeneration and functional recovery in a rat model of sciatic nerve injury. Used as an ex vivo drug selectable marker in gene-modified primary bone marrow stromal cells for cytidine deaminase assay.
Physical Appearance: White Powder
Optical Rotation: +34.6 ° (Lit.)
UV/Visible Absorbance: λ max (water): 270 - 272 nm
Solubility: Soluble in water (50 mg/mL- clear colorless to faint yellow solution)

Supplier: MP Biomedicals

Description: Use of dextrans as long and hydrophilic spacer arms improves the performance of immobilized proteins acting on macromolecules. Dextrans are used in many applications as platelet aggregants, plasma volume extenders, osmotic pressure regulators, stabilizers, organ separation media, matrix components, copolymers, microcarriers, binding agents, viscosity modifiers, antithrombotics, lubricants and physical structure components. They may be used as long hydrophilic spacer arms to improve the performance (freedom of movement) of conjugated/bound proteins. Dextrans may be derivatized for use in biosensor systems.
Dextran is branched polysaccharide made up of linear α (1→6) linked glucose units and α (1→3) link initiated branches; it ranges in size from 10,000 to 150,000 Kd.Depending upon its molecular weight it has different uses in different fields.
Physical Appearance: White powder
Solubility: Very soluble in water (50 mg/mL), slightly soluble in alcohol

Certificates

Catalog Number: (75983-036)

Supplier: MP Biomedicals

Description: Fluorescein-Conjugated Goat F(ab')2 Fragment to Human Complement C3 is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat F(ab')2 fragment to human complement C3 and buffer salts.

Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacteria infection.

The total protein is measured using the Biuret procedure with bovine albumin as the standard; F/P ratio is calculated using absorbance at 491.5nm. Antibody titer of the IgG fraction is standardized by equivalence-point precipitation. The F(ab')2 fragments are tested for purity and specificity at a minimum of 20 mg/mL using immunoelectrophoresis.

Certificates

Supplier: MP Biomedicals

Description: Replacement consumables for SafTest Platforms.

Supplier: MP Biomedicals

Description: Obtain rapid growth of <i>E. coli</i> and high yields of plasmid DNA past log phase.

Certificates

Supplier: MP Biomedicals

Description: o-Dianisidine is a peroxidase substrate suitable for use in ELISA procedures.

Catalog Number: (IC0210128901)

Supplier: MP Biomedicals

Description: Casein is the name for a family of related phosphoproteins (αS1, αS2, β, κ). These proteins are commonly found in mammalian milk, making up 80% of the proteins in cow milk and between 20% and 45% of the proteins in human milk. Casein has a wide variety of uses, from being a major component of cheese, to use as a food additive, to a binder for safety matches. As a food source, casein supplies amino acids; carbohydrates; and two inorganic elements, calcium and phosphorus.

Certificates

Supplier: MP Biomedicals

Description: p-Nitrophenol is used as an indicator in 0.1% alcohol solutions. 4-Nitrophenol is used as a precursor to prepare phenetidine, acetophenetidine and pH indicator. Its carboxylate ester derivatives are involved as an active component for the construction of amide moieties in peptide synthesis.

Certificates

Catalog Number: (IC113002321)

Supplier: MP Biomedicals

Description: Rich medium for the growth of <i>E. coli </i>and certified for plasmid and other bacterial cultures in liquid medium.

Supplier: MP Biomedicals

Description: DL-Dithiothreitol is a Clelands reagent; Protective agent for sulfhydryl groups (-SH). Quantitatively reduces disulfides (-S-S- to -SH). In this reaction the DTT is oxidized to the cyclic disulfide which ensures the reduction of other disulfides in solution. Disulfide reduction occurs quickly at pH 8.

SDS

Supplier: MP Biomedicals

Description: Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Supplier: MP Biomedicals

Description: Tunicamycin is a mixture of Tunicamycins A, B, C and D. It is a nucleoside antibiotic active in vitro against Gram-positive bacteria, yeasts, fungi, and viruses.

Catalog Number: (76102-638)

Supplier: MP Biomedicals

Description: Melphalan is an antineoplastic agent.