The Chemilum de Lys® HDAC/SIRT Chemiluminescent Drug Discovery Kit give you all the benefits of chemiluminescent detection without any of the side effects.
The HDAC/SIRT Chemiluminescent Drug Discovery Kit is a complete assay system designed to measure histone deacetylase (HDAC) and sirtuin activity in cell or nuclear extracts, immunoprecipitates or purified enzymes. It comes in a convenient 96-well format, with all reagents necessary for chemilumnescent HDAC activity measurements and calibration of the assay. In addition, a HeLa nuclear extract, rich in HDAC activity, is included with the kit. The extract is useful as either a positive control or as the source of HDAC activity for inhibitor/drug screening. Also included are Trichostatin A and Nicotinamide, which may be used as model inhibitors for HDACs and sirtuins, respectively.
The HDAC Chemiluminescent Activity Assay is based on the unique Chemilum de Lys® Substrate and Developer combination. The Chemilum de Lys® system (Chemiluminescent Histone deAcetylase Lysyl Substrate/Developer) is a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. The assay procedure has three steps (Fig. 1). First, the Chemilum de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (HeLa nuclear or other extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Chemilum de Lys® Developer followed by Enhancer produces light. The reaction is luciferase-free.
Ordering information: Kit contains Nuclear Extract from HeLa Cells, Chemilum de Lys® Substrate, Chemilum de Lys® Developer Concentrate, Trichostatin A (HDAC Inhibitor), NAD (Sirtuin Substrate), Nicotinamide, HDAC Assay Buffer, Developer Buffer, Chemilum de Lys® Enhancer part A, Chemilum de Lys® Enhancer part B, 1/2 volume white microplate.
