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You Searched For: Protein Assays

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.


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Product Image-97064-924

VWR® Bradford Method Protein Assay Kit

Supplier: VWR
Description: The Bradford Protein Assay is the preferred colorimetric assay for quantifying total protein concentration. Based upon complex formation between basic and aromatic amino acid residues with Coomassie® Brilliant Blue G-250 dye, the Bradford method is easier, faster, and more sensitive than the Lowry method. The standard protocol is used for measuring 10-100 μg of protein and the microassay is used for measuring 1-10 μg of protein. For maximum convenience, Bradford Reagent and 0.5 mg/ml BSA standard solution are offered individually or as components of a kit.

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AzureRed Fluorescent Protein Stain, Azure BioSystems

Catalog Number: (10147-342)
Supplier: Azure Biosystems
Description: AzureRed Fluorescent Protein Stain is a quantitative total protein stain for blots and blots with the sensitivity to detect less than 1 ng of protein per band or spot

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Auto-Ubiquitinylation Kit, Enzo Life Sciences

Catalog Number: (89165-248)
Supplier: Enzo Life Sciences
Description: This E3 ligase auto-ubiquitinylation kit enables proteins to be tested for ubiquitin E3 ligase activity through assessment of their ability to under auto-ubiquitinylation
Product Image-89156-498

DPPIV/CD26 Assay Kit for Biological Samples, Enzo Life Sciences

Catalog Number: (89156-498)
Supplier: Enzo Life Sciences
Description: The DPPIV/CD26 Assay kit for biological samples is a complete assay system designed to measure DPPIV activity in biological fluids such as plasma, serum, urine, and saliva. Uses for the kit include correlation of DPPIV activity with disease states or determination of the efficacy of DPPIV inhibitors administered <i>in vivo</i>.
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QBT Fatty Acid Uptake Assay Kit, Molecular Devices

Supplier: Molecular Devices
Description: The QBT™ Fatty Acid Uptake Assay is a homogeneous assay amenable to high-throughput screening.

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eFluxx-ID® Multidrug Resistance Assay Kit, Enzo Life Sciences

Supplier: Enzo Life Sciences
Description: The EFLUXX-ID® Multidrug Resistance Assay Kit allows for functional detection of all three clinically relevant ABC transporter proteins MDR1 (p-glycoprotein), MRP1/2, and BCRP

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Foxp3 / Transcription Factor Staining Buffer Kit, Tonbo Biosciences

Supplier: Tonbo Biosciences
Description: Tonbo Biosciences Foxp3 / Transcription Factor Staining Buffer Kit contains specially formulated buffers and solutions for optimal resolution and low background in your analysis of nuclear antigens by flow cytometry

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p70 S6K Activity Kit, Enzo Life Sciences

Catalog Number: (89141-850)
Supplier: Enzo Life Sciences
Description: For the measurement of p70 S6K activity in partially purified, purified, or crude enzyme preparations from any species.
Product Image-10033-580

Chemilum de Lys® HDAC/SIRT Chemiluminescent Drug Discovery Kit, Enzo Life Sciences

Catalog Number: (10033-580)
Supplier: Enzo Life Sciences
Description: The Chemilum de Lys® HDAC/SIRT Chemiluminescent Drug Discovery Kit give you all the benefits of chemiluminescent detection without any of the side effects.
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Ubiquitinylation Kit, Enzo Life Sciences

Catalog Number: (89165-452)
Supplier: Enzo Life Sciences
Description: Versatile tool for generation of ubiquitin-E2 thioesters.
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ADAM17 Fluorometric Drug Discovery Kit, Enzo Life Sciences

Catalog Number: (76002-366)
Supplier: Enzo Life Sciences
Description: Designed to screen ADAM17 (TACE) inhibitors using a quenched fluorogenic peptide. The ADAM17 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen ADAM17 (TACE) inhibitors using a quenched fluorogenic peptide: Mca-PLAQAV-Dpa-RSSSR-NH2. Mca fluorescence is quenched by the Dpa group until cleavage by proteases separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of ADAM17, a potential therapeutic target. The compound GM6001 is also included as a prototypic control inhibitor.
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Ubiquitin Conjugating Kit (HeLa Lysate-Based), Enzo Life Sciences

Catalog Number: (89165-450)
Supplier: Enzo Life Sciences
Description: 1. Ubiquitinylation of specific endogenous HeLa lysate proteins, followed by their immediate detection/analysis using antibodies to the protein/s of interest, indicating a particular protein is a substrate for the ubiquitin-proteasome pathway. 2. Ubiquitinylation of proteins of interest contained in exogenously added expression culture/cell extracts or tissue lysates/extracts, followed by their immediate detection/analysis or isolation/purification for use in subsequent experiments. 3. Modification of proteins using ubiquitin derivatives or mutants for improved detection/analysis or investigation of alternative (non-proteasomal) ubiquitin related pathways in subsequent experiments. For example, biotinylated-ubiquitin (Prod. No. BML-UW8705), for sensitive detection
Product Image-76002-532

PROTEOSTAT® Thermal Shift Stability Assay Kit, Enzo Life Sciences

Supplier: Enzo Life Sciences
Description: This kit has been designed for monitoring protein stability under systematic thermal stress conditions.

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FLUOR DE LYS® SIRT5 fluorometric drug discovery assay kit, Enzo Life Sciences

Catalog Number: (10033-576)
Supplier: Enzo Life Sciences
Description: The SIRT5 Fluorometric Drug Discovery Kit is a sensitive fluorometric assay is designed to measure the lysyl desuccinylase activity of the recombinant human SIRT5.
Product Image-89156-536

Caspase-1 Assay Kit for Drug Discovery, Enzo Life Sciences

Catalog Number: (89156-536)
Supplier: Enzo Life Sciences
Description: This kit is a complete assay system to measure protease activity of recombinant caspase-1
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HAT Activity Assay Kit, Enzo Life Sciences

Catalog Number: (89156-146)
Supplier: Enzo Life Sciences
Description: Histone acetyltransferases (HATs) have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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