HAT Activity Assay Kit, Enzo Life Sciences

Supplier: Enzo Life Sciences

HAT Activity Assay Kit, Enzo Life Sciences

HAT Activity Assay Kit, Enzo Life Sciences

850-326-KI01

89156-146EA 827 USD

89156-146

HAT Activity Assay Kit, Enzo Life Sciences

Assays Protein Assays

Histone acetyltransferases (HATs) have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation

The kit utilizes active recombinant HAT as a positive control and acetyl-CoA as a cofactor. Acetylation of peptide substrate by active HAT releases the free form of CoA which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous and suitable for kinetic studies. The kit provides a simple, straightforward protocol for a complete assay.

HAT Activity Colorimetric Assay Kit offers a convenient, nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples.

Delivery information: Kit includes 2X HAT Assay Buffer, HAT Substrate I, HAT Substrate II, NADH Generating Enzyme, NE (Nuclear Extract 4 mg/mL), HAT Reconstitution Buffer.

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