You Searched For: Urea


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Supplier: MP Biomedicals
Description: Urea is a prinicipal protein metabolite end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle and the major product for the removal of free ammonia (NH4+) in vivo.

Supplier: Thermo Scientific Chemicals
Description: Used as a protein denaturant
Supplier: MP Biomedicals
Description: Urea is a mild agent usually used in the solubilization and denaturation of proteins.

SDS

Supplier: MP Biomedicals
Description: Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with 6 M guanidine hydrochloride such as inclusion bodies; and in the extraction of the mitochondrial complex.
Supplier: TCI America
Description: [for Biochemical Research]
CAS Number: 57-13-6
MDL Number: MFCD00008022
Molecular Formula: CH4N2O
Molecular Weight: 60.06
Purity/Analysis Method: >99.0% (N)
Form: Crystal
Color: White
Melting point (°C): 135
Supplier: Thermo Scientific Chemicals
Description: Urea 99.5% for molecular biology DNase-, RNase-, protease-free

Supplier: MP Biomedicals
Description: Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-Urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L Urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M Urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat Urea containing buffers above 37 °C

Supplier: Thermo Scientific Chemicals
Description: Urea 99+% for biochemistry

Supplier: Thermo Scientific Chemicals
Description: MDL: MFCD00008022 Beilstein Registry No.: 635724 Fieser: 1,1262 2,455
Supplier: Thermo Scientific Chemicals
Description: Urea 99% ACS

Supplier: Thermo Scientific Chemicals
Description: Urea 99.5% for analysis

Supplier: Thermo Scientific Chemicals
Description: Urea 98%, pearls, Extra Pure

Catalog Number: (95017-130)
Supplier: Cytiva
Description: 500 g

Catalog Number: (80058-810)
Supplier: MilliporeSigma
Description: (Carbimide; carbonyl diamide). White solid. PROTECT FROM MOISTURE. End product of the urea cycle. A topical keratolytic agent. Soluble in H2O. RTECS YR6250000, CAS 57-13-6, M.W. 60.1. WARNING! May be carcinogenic/teratogenic.

Supplier: MilliporeSigma
Description: Ideal for protein unfolding/denaturation.
Supplier: Spectrum Chemical Mfg. Corp.
Description: Urea, BiotechGrade is an odorless and colorless solid that is an important nitrogen-containing substance found in mammal urine. In concentrations up to 10 M, Urea is a powerful protein denaturant as it disrupts the noncovalent bonds in the proteins. This property can be exploited to increase the solubility of some proteins. Spectrum offers highly pure reagents suitable for biochemical research and analysis. The critical parameters involved are absence of inhibitors such as traces of heavy metals as well as biochemical function tests for enzymes, coenzymes and enzyme substrates.

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