ECL Substrate
Which Azure substrate is right for you?
| Azure Substrate | Radiance™ Substrate | Radiance™ Plus Substrate |
| Use | Extended dynamic range for detecting low and high abundance proteins within the same blot | Low abundance protein detection |
| Sensitivity | Mid femtograms | Low femtograms |
| Signal Duration | 10-24 hours | 6-8 hours |
| Compare Performance to | Amersham™ ECL™ Prime SiperSignal® West Dura® | Amersham™ ECL™ Select SuperSignal® West Femto® |
Radiance™ Substrate
Increase the sensitivity and quantitative accuracy of your chemiluminescent Western blots. Specially developed for CCD imaging, Radiance produces a strong, long-lasting signal with extremely low background, perfect for detecting low abundance proteins. Because the substrate is not depleted at high protein loads, Radiance provides the largest dynamic range of any chemiluminescent substrate, improving quantitative accuracy.
Radiance™ Plus Substrate
Radiance Plus is the most sensitive HRP substrate available from Azure for chemiluminescent Western blotting. With attomole sensitivity and a long-lasting signal, Radiance Plus allows you to detect bands not visualized with other substrates. High signal-to-noise and a large dynamic range make it ideal for quantifying low-intensity bands.