Single-Use RED (rapid equilibrium dialysis) Plate is composed of disposable high-density polypropylene and is pre-loaded with 48 equilibrium dialysis membrane inserts. Each insert is comprised of two side-by-side chambers separated by an O-ring-sealed vertical cylinder of dialysis membrane with varying molecular-weight cutoffs (8 or 12 kD MWCO).
- Easy and ready to use
- Design enables equilibrium to reach 200 min⁻¹ agitation with in three to four hours
- Reproducible and accurate for plasma binding assays
- Robust compartmentalised design eliminates potential for leakage
- For guaranteed performance each lot of inserts is functionally tested in a protein-binding assay
RED device is specifically designed and extensively validated for plasma serum binding assays.
This format requires no extensive assembly steps or specialized equipment, and each chamber/well is easily accessible from the top of the device. The base plate has a standard 96-well plate footprint with 9×9 mm well spacing. Additionally, the high membrane surface-to-volume ratio allows rapid dialysis, where equilibrium can be reached in 4 hours with high levels of reproducibility and accuracy.
In addition to plasma protein binding, the device is used for determining drug partition between red blood cell and plasma; protein binding of liver microsomes to improve the correlation between in vitro and in vivo intrinsic clearance; and the competition between tissue protein binding against plasma proteins. The single-use RED plate is validated for minimal nonspecific binding.
