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Catalog Number: (PI30030)
Supplier: Invitrogen
Description: Dulbecco's Modified Eagle Medium (DMEM) is for supporting and maintening a broad spectrum of mamamalian cell types.

Catalog Number: (PI53149)
Supplier: Invitrogen
Description: UltraLink™ Hydrazide resin is used to prepare affinity columns with antibodies or other glycolsylated proteins.

Supplier: Invitrogen
Description: Frozen liquid, 100uL 20X 1 vial with thermolysin isolate and culture highly viable functional primary cardiomyocytes.
Catalog Number: (PI78436)
Supplier: Invitrogen
Description: Pepstatin A Protease Inhibitor reversably inhibits aspartic acid proteases.

Catalog Number: (PIN502)
Supplier: Invitrogen
Description: ELISA Blocker Blocking Buffer is a protein buffer that effectively blocks plate binding sites that remain in polystyrene microplate wells following initial coating steps in ELISA procedures. The ready-to-use buffer is optimized and routinely used for sandwich ELISA procedures involving cytokines, such as many of the Thermo Scientific Pierce ELISA Kits.

Supplier: Invitrogen
Description: Thermo Scientific Pierce DSG is a water-insoluble, homo-bifunctional N-hydroxysuccinimide ester (NHS-ester) crosslinker often used for conjugating radiolabeled ligands to cell-surface receptors. The NHS ester is the simplest and most commonly used reactive group for crosslinking and labeling proteins and peptides. NHS esters react with primary amines on the N-termini of peptides and the  amine of lysine residues, forming a stable, covalent amide bond and releasing the NHS group.
Catalog Number: (PIN503)
Supplier: Invitrogen
Description: ELISA Wash Buffer is a Tris-based wash buffer for use in cytokine and other sandwich ELISA procedures. The buffer is the same formulation that is supplied with most Thermo Scientific Pierce ELISA Kits for cytokines and chemokines. The 30X concentrated stock solution is easily diluted 1:30 (vol:vol) in ultrapure water to yield sufficient wash buffer for a typical 96-well microplate ELISA protocol. Supplement an existing ELISA Kit or use as a convenient general purpose wash buffer for customized ELISA methods.

Supplier: Invitrogen
Description: Thermo Scientific Pierce BS3 (Sulfo-DSS) is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.
Supplier: Invitrogen
Description: Thermo Scientific™ Pierce™ Glutathione Magnetic Agarose Beads provide a fast, convenient method for purification of glutathione-S-transferase (GST) from a bacterial, yeast, or mammalian crude cell lysate.
Catalog Number: (PI88438)
Supplier: Invitrogen
Description: Thermo Scientific Heavy and Light Amino Acids are used to specifically analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) quantification kits.

Catalog Number: (PI21277)
Supplier: Invitrogen
Description: The Pierce™ His Protein Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with His-tagged fusion proteins.

Supplier: Invitrogen
Description: Thermo Scientific Pierce DSP (Lomant's Reagent) is a water-insoluble, homo-bifunctional N-hydroxysuccimide ester (NHS ester) crosslinker that is thiol-cleavable, primary amine-reactive, and useful for many applications. DSP contains an amine-reactive NHS ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7–9 to form stable amide bonds and releasing N-hydroxy-succinimide. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS ester crosslinking reagents. DSP is non-sulfonated and insoluble in water, so it must first be dissolved in an organic solvent and then added to the aqueous reaction mixture. Because DSP does not possess a charged group, it is lipophilic and membrane-permeable and so useful for intracellular and intramembrane conjugation. A sulfonated analog of DSP (DTTSP) is water soluble. DSS, the non-cleavable analog of the DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.
Catalog Number: (PI24585)
Supplier: Invitrogen
Description: The Thermo Scientific Pierce Reversible Protein Stain Kit for PVDF Membranes is a rapid and sensitive alternative to Ponceau S stain for protein detection on PVDF membrane after transfer from polyacrylamide gels.

Catalog Number: (PI62249)
Supplier: Invitrogen
Description: Hoechst 33342 fluorescent stain is a high-quality solution of Hoechst dye for fixed- and live-cell fluorescent staining of DNA and nuclei in cellular imaging techniques.

Catalog Number: (PI17888)
Supplier: Invitrogen
Description: Thermo Scientific Pierce Sodium Acetate Solution is a sterile, 3M solution of sodium acetate that is useful for basic methods of DNA precipitation, DNA purification and storage.

Supplier: Invitrogen

SDS

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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