Immunomagnetic isolation of mouse naïve CD4+ T cells.
- Fast and easy-to-use
- Up to 97% purity
- No columns required
Isolate highly purified naïve CD4+ T cells from single-cell suspensions of splenocytes or other tissues by using a simple, two-step method. First, naive CD4+ T cells are pre-enriched by negative selection using the EasySep™ Mouse Naïve CD4+ T Cell Pre-Enrichment Cocktail containing biotinylated antibodies directed against non-naïve CD4+ T cells (CD8, CD11b, CD11c, CD19, CD24, CD25, CD45R, CD49b, TCRγ/δ, TER119). Then CD4+CD62L+ cells, pre-labeled with CD62L PE, are selected using the EasySep™ PE Selection Cocktail. The desired cells are labeled with antibodies and magnetic particles. The cells are separated without columns using an EasySep™ magnet.
For the isolation of mouse naïve CD4+ T cells in as little as 15 minutes by negative selection, we recommend using the EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (19765).
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