NEBridge Ligase Master Mix, New England Biolabs

Supplier: New England Biolabs (NEB)

NEBridge Ligase Master Mix, New England Biolabs

NEBridge Ligase Master Mix, New England Biolabs

M1100S M1100L

76538-670EA 97 USD

76538-670 77025-080

NEBridge Ligase Master Mix, New England Biolabs

Nucleic Acid Reagents Cloning Kits and Ligation

NEBridge Ligase Master Mix is a 3X master mix for Golden Gate Assembly. Designed for use with NEB Type IIS restriction enzymes, this master mix contains T4 DNA Ligase in an optimized reaction buffer with a proprietary ligation enhancer.

Optimized for efficient and accurate Golden Gate Assembly
Convenient 3X Master Mix formatUse with NEB Type IIS Restriction Enzymes
Use for seamless cloning –no scar remains following assembly
Ideal for ordered assembly of multiple fragments (2 to 50+) in a single reaction

Users need only choose their preferred NEB Type IIS restriction enzyme and add DNA substrates to be assembled. Low complexity single fragment insertions, as well as moderate complexity (3 to 6 fragment) and high complexity (7 to 25 fragment) assemblies, are all supported with this optimized reagent and accompanying protocols.

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