You Searched For: Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Catalog Number: (99902-306)
Supplier: Agilent Technologies
Description: <i>Pfu</i> DNA ligase is a recombinant enzyme derived from <i>Pyrococcus furiosus</i> hyperthermophilic marine archaebacterium that catalyzes linkage of adjacent 5'-phosphate and 3'-hydroxy ends of double-stranded DNA at 45 to 80 °C, with a temperature optimum near 70 °C for nick-sealing reactions.


Supplier: Rockland Immunochemical
Description: DNA Ligase T4 Molecular Biology Grade

Supplier: Agilent Technologies
Description: The GeneMorph II random mutagenesis kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis.
Supplier: Agilent Technologies
Description: The StrataClone PCR Cloning Kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase.

Supplier: TELESIS BIO
Description: Generate seamless plasmid DNA or BAC clones without restriction digestion.

SDS

Supplier: TELESIS BIO
Description: The Select DNA cloning Golden Gate Assembly BsmBI kits are compatible with the Type IIS restriction enzymes BsmBI. Please choose the appropriate kit based on the number of fragments being assembled.

New Product

Catalog Number: (76299-644)
Supplier: New England Biolabs (NEB)
Description: The NEB Cloning Competent E.coli Sampler allows to sample four of popular chemically competent strains.


Supplier: TELESIS BIO
Description: The Select DNA cloning and amplification kits generate a minimum of 10 µg of amplified DNA from fragments of 0.3 to 1.8 kb using the BioXp® 3250.

New Product

Supplier: TELESIS BIO
Description: BioXp Select plasmid amplification kits contain reagents for isothermal amplification of plasmid DNA.

New Product

Supplier: New England Biolabs (NEB)
Description: The Quick Blunting Kit is used to convert DNA with incompatible 5´ or 3´ overhangs to 5´ phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors

Small Business Enterprise

Catalog Number: (102877-564)
Supplier: New England Biolabs (NEB)
Description: This PCR Cloning Kit contains an optimized 2X Cloning Master Mix with a proprietary ligation enhancer and a linearized vector that uses a novel mechanism for background colony suppression to give a low background.

SDS Small Business Enterprise


Supplier: TELESIS BIO
Description: The Select DNA cloning Gibson assembly kits can generate Gibson clones starting with fragments and vector supplied by the end user.

New Product

Catalog Number: (102969-096)
Supplier: New England Biolabs (NEB)
Description: This PCR Cloning Kit contains an optimized Cloning Mix containing a proprietary ligation enhancer and a linearized vector that uses a novel mechanism for background colony suppression to give a low background.

Small Business Enterprise


Supplier: TELESIS BIO
Description: Generate seamless plasmid DNA or BAC clones without restriction digestion.

SDS

Supplier: TELESIS BIO
Description: BioXp Select plasmid amplification kits contain reagents for isothermal amplification of plasmid DNA.

New Product

Supplier: TELESIS BIO
Description: The Select DNA cloning and amplification kits generate a minimum of 10 µg of amplified DNA from fragments of 0.3 to 1.8 kb using the BioXp 9600.

New Product

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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