DNase I

Supplier: Agilent Technologies
600032 600031 600032 600031
ST600032EA 354.88 USD
ST600032 99902-262 CAST600032 CA99902-262
DNase I
Enzymes
Purified DNase I tested to be free of contaminating activity for the degradation of nucleic acids.

  • RNase Free
  • Contains a mixture of four isoenzymes from bovine pancreas (10 units/µL)

DNase I, RNase Free catalyzes the degradation of double-stranded DNA into oligonucleotides 1,2 and mononucleotides. This enzyme has been isolated as a mixture of four isoenzymes from bovine pancreas that cut preferentially next to pyrimidine nucleotides.

Reaction Conditions: 40 mM Tris-HCl (pH 7.5), 6 mM MgCl2, 2 mM CaCl2, 1 µg DNA and 1 unit enzyme in a 25 µL volume. Incubate 30 minutes at 37 °C.

Caution: For research use only. Not for use in diagnostic procedures.
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