Prostaglandin Screening ELISA Kit, Cayman Chemical Company

Supplier: Cayman Chemical Company

514012-480 514012-96
75816-872EA 1502.49 USD
75816-872 75816-874
Prostaglandin Screening ELISA Kit, Cayman Chemical Company
Assays ELISAs

This kit is used for the quantification of total PG levels in tissue culture medium.


  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure total PG levels down to 30 pg/ml
  • Read: Colorimetric at 405 to 420 nm


This assay was developed for screening applications in which the relative amount of prostaglandin (PG) production for a large number of cell culture samples must be determined. Our PGE2 assay (Item No.514010) is unparalleled in sensitivity and specificity, but the steep standard curve can make screening many samples tedious.


The specificity of the PGE2 assay also eliminates quantification of other PGs which may be produced by the cells. The antiserum used in this assay exhibits high cross reactivity for most PGs which will allow quantification of all the PGs in a given sample with a single assay.


The broad standard curve minimizes the need for sample dilution. Because this assay will also recognize thromboxane B2 (TXB2), cell culture media must not contain fetal calf serum (FCS). The high concentration of TXB2 in serum (approximately 400 ng/ml) will completely displace the tracer, making PG measurement impossible.


Delivery information: Kit contains antiserum, tracer, standard, ELISA and wash buffer concentrate, polysorbate 20, plate and cover sheet, Ellman's reagent, tracer and antiserum dye.

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SPECIFICATIONS

Environmentally Preferable
Primary antibody reactivity
Host
Sensitivity 30 pg/ml (80% B/B0)
Detection Range 15.6 - 2000 pg/ml
Mid Point 125 - 250 pg/ml (50% B/B0)
Developmental Time 60 - 90 min
Incubation Time 18 hr
Storage Temperature –20 °C

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