You Searched For: Cayman Chemical Company

Supplier: Cayman Chemical Company

Description: A novel method to increase MS sensitivity for detection of lipids.

Supplier: Cayman Chemical Company

Description: Wedelolactone, Purity >/=98%, CAS 524-12-9, MF: C16H10O7, MW: 314.3, Synonyms: IKK Inhibitor II, 1,8,9-trihydroxy-3-methoxy-6H-benzofuro[3,2-c][1]benzopyran-6-one, natural coumestan originally isolated from plants used in herbal therapies, Size: 10mg

Catalog Number: (76293-162)

Supplier: Cayman Chemical Company

Description: STING A162 variant (human recombinant) contains amino acids 138-379 of the wild-type variant (R232) with an alanine substituted for serine at position 162. Stimulator of interferon genes (STING) is a component of the innate immune response that binds to cyclic dinucleotides, which are bacterial second messengers, leading to activation of NF-κB and transcription of immunomodulatory genes, including type I interferon (IFN). The R232 variant of STING is the most common variant in the human population, found at a frequency of 57.9% in the 1000 Genome Project. The SNP variant H232 is found at a 13.7% frequency. The S162A point mutation is located in the cyclic dinucleotide binding site of the human STING variants R232 and H232 and allows human STING to bind to DMXAA, a compound previously known to bind mouse, but not human, STING. When STING S162A is bound to DMXAA, it adopts the closed conformation, similar to the conformation it has when bound to the second messenger 2’3’-cGAMP (Item No. 19887), and activates the IFN pathway similarly to mouse STING.

Catalog Number: (76293-120)

Supplier: Cayman Chemical Company

Description: Cyclic GMP-AMP (cGAMP) synthase (cGAS; Item No. 22810) is a nucleotidyltransferase located in the cytosol that acts as a cytosolic DNA sensor to detect foreign DNA from microbial pathogens as part of the innate immune response. Upon binding to cytosolic DNA, cGAS produces the cyclic dinucleotide second messenger cGAMP, which activates stimulator of interferon genes (STING), leading to activation of the type I interferon (IFN) pathway. <em>In vitro</em>, fibroblasts, macrophages, and dendritic cells isolated from cGAS knockout (cGAS^-/-) mice do not produce type I IFNs following DNA transfection or DNA virus infection.{39163} Similarly, cells containing a frame-shift mutation in the cGAS locus fail to mount an immune response to HIV and other retroviruses.{39164} <em>In vivo</em>, cGAS^-/- mice infected with herpes simplex virus 1 (HSV-1) have lower levels of IFN-α and IFN-β, shorter survival times, and higher post-mortem levels of HSV-1 in the brain. Cayman’s cGAS Monoclonal Antibody (Clone 5G10) recognizes the full length human cGAS protein at ~59 kDa.

Catalog Number: (76292-900)

Supplier: Cayman Chemical Company

Description: TANK-binding kinase 1 (TBK1) is a noncanonical inhibitor of NF-κB kinase (IKK) that has an essential role in regulating inflammatory responses to pathogens. Following activation of toll-like receptors by viral DNA, TBK1 interacts with various partners such as STING, MAVS, and TANK to phosphorylate and activate interferon regulatory factors (IRFs) 3 and 7 as well as DEAD-box helicase 3 X-linked (DDX3X), which leads to transcriptional activation of pro-inflammatory and antiviral genes including interferon (IFN) subtypes α and β. TBK1 induces nuclear translocation of NF-κB to initiate a pro-inflammatory response <em>via</em> phosphorylation of NF-κB inhibitor α (NFκBIα), IKK2, or NF-κB p65 subunit (RELA). Cytosolic localization of <em>E. coli</em>, <em>Salmonella</em>, and <em>S. pyogenes</em> increases in TBK1^-/- murine embryonic fibroblasts, macrophages, and epithelial cells, suggesting TBK1 maintains vacuolar integrity that is critical to bacterial clearance. TBK1 also phosphorylates the autophagy receptor optineurin to enhance binding of ubiquitin-like microtubule-associated protein light chain 3 (LC3) modifiers and induce autophagic clearance of <em>S. enterica</em>.

Catalog Number: (76292-908)

Supplier: Cayman Chemical Company

Description: Nuclear Factor-κB (NF-κB) is sequestered in the cytoplasm by the IκB family of inhibitory proteins that mask the nuclear localization signal of NF-κB, thereby preventing translocation of NF-κB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines result in phosphorylation and degradation of IκB, releasing NF-κB dimers. NF-κB dimers subsequently translocate to the nucleus and activate target genes. Synthesis of IκBα is autoregulated. IκB proteins are phosphorylated by IκB kinase complex consisting of at least three proteins, IKK1/α, IKK2/β, and IKK3/γ. IKKγ preferentially interacts with IKKβ and is required for activation of IKK complex. IKKγ is also known as NF-κB essential modulator (NEMO). The human T-cell leukemia virus type I Tax oncoprotein that activates NF-κB binds neither to IKKα nor IKKβ, but complexes directly with IKKγ. This suggests that IKKγ may be a key molecule acting as an adapter for oncoprotein specific signaling to IKKα and IKKβ.

Supplier: Cayman Chemical Company

Description: ML RR-S2 CDA is a synthetic cyclic dinucleotide (CDN) that contains non-canonical 2'5'-phosphodiester bonds and is an activator of stimulator of interferon genes (STING).1 It contains mixed linkages (ML) with both 2'5' and 3'5' linkages, which leads to increased thermal stability of human STING in a differential scanning fluorimetry (DSF) assay. ML RR-S2 CDA increases type I interferon production by THP-1 human monocytes relative to unmodified cyclic di-AMP (CDA; Item No. 17753), indicating the ML enhances its action at human STING. It induces expression of IFN-β and the pro-inflammatory cytokines TNF-α, IL-6, and Mcp-1 in murine bone marrow macrophages (BMM) isolated from wild-type, but not STING-/-, mice. ML RR-S2 CDA also induces IFN-β expression in peripheral blood mononuclear cells (PBMCs) isolated from donors carrying STINGWT/WT, STINGWT/REF, and STINGWT/HAQ alleles. In vivo, ML RR-S2 CDA initiates tumor regression and prevents tumor growth upon tumor cell reimplantation in 4T1 breast and CT26 colon cancer mouse xenograft models.

Supplier: Cayman Chemical Company

Description: PPM-18, Purity >/=95%, CAS 65240-86-0, MF: C17H11NO3, MW: 277.3, Synonyms: NSC 73233, N-(1,4-dihydro-1,4-dioxo-2-naphthalenyl)-benzamide, naphthoquinone compound that inhibits NF-KB activation in vitro and in vivo, Formulation: A crystalline solid, Size: 25mg

Supplier: Cayman Chemical Company

Description: Echinocandin B inhibits 1,3-β glucan synthase; inhibits incorporation of radiolabeled glucose into alkali-insoluble glucan by <i>S. cerevisiae</i> spheroplasts; inhibits growth of <i>C. albicans in vitro</i> (MIC = 3.9 µg/ml after 2 days)

Supplier: Cayman Chemical Company

Description: A competitive assay for measurement of enterolactone in plasma, urine, and other biological sample matrices.

Catalog Number: (75816-742)

Supplier: Cayman Chemical Company

Description: A 192-well assay for measurement of cholesterol.

Catalog Number: (75816-690)

Supplier: Cayman Chemical Company

Description: A sensitive, non-radioactive method for detecting HIF-1α from whole cell lysates.

Catalog Number: (75817-098)

Supplier: Cayman Chemical Company

Description: 96-Well assay for measurement of Oct4.

Supplier: Cayman Chemical Company

Description: For the quantification of 11-keto testosterone in plasma and other sample matrices.

Supplier: Cayman Chemical Company

Description: For the quantification of testosterone in serum, plasma, tissue culture supernatants and other sample matrices.

Catalog Number: (75817-102)

Supplier: Cayman Chemical Company

Description: Neutrophil/Monocyte Respiratory Burst Assay Kit provides PMA, dihydrorhodamine 123, and additional reagents necessary for inducing and quantifying a respiratory burst response in neutrophils and monocytes by flow cytometry. The assay can be performed on whole blood or on cells in various types of cell culture media. Because the assay reagents are not species-specific, this assay can be used in any species or cell type capable of producing a NADPH oxidase-dependent respiratory burst response.