5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (5/6-FAM SE)

Supplier: Anaspec Inc

Synonyms: 5(6)-FAM SE , NHS-Fluorescein , FLUOS

AS-81006-1000 AS-81006-100 AS-81006

103010-772EA 1710 USD

103010-772 103010-768 103010-770

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (5/6-FAM SE)

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (5/6-FAM SE)

Although FITC reagents have been more often used to prepare fluoresceinated bioconjugates, the low stability of FITC bioconjugates makes some researchers use amine-reactive succinimidyl esters of carboxyfluorescein (commonly called FAM) in bioconjugations. FAM reagents give carboxamides that are more resistant to hydrolysis. We have shown that FAM reagents require less stringent reaction conditions and give better conjugation yields, and the resulted conjugates have superior stability. We noted that FITC labeled peptides tend to deteriorate more quickly than the corresponding FAM conjugates.

Spectral properties: Abs/Em = 494/519 nm
Solvent system: DMF or DMSO
Appearance: Orange Powder
Solubility: 1 mmol in 1ml DMF
Storage Temperature: -20°C desiccated and protected from light
Shipping condition: Blue ice

Formula: C₂₅H₁₅NO₉
MW: 473.39 g/mol
Storage Temperature: Freezer

MDL Number: MFCD04119713
CAS Number: 117548-22-8

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