5(6)-Carboxyfluorescein

Supplier: Anaspec Inc

Synonyms: 3′,6′-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthene]-ar-carboxylic acid , 5(6)-FAM

AS-81002-25 AS-81002-1 AS-81002 AS-81002-10

103010-750EA 439.92 USD

103010-750 103010-752 103010-746 103010-748

5(6)-Carboxyfluorescein

5(6)-Carboxyfluorescein

Although FITC reagents have been more often used to prepare fluoresceinated bioconjugates, the low stability of FITC bioconjugates makes some researchers use amine-reactive succinimidyl esters of carboxyfluorescein (commonly called FAM) in bioconjugations. FAM reagents give carboxamides that are more resistant to hydrolysis. We have shown that FAM reagents require less stringent reaction conditions and give better conjugation yields, and the resulted conjugates have superior stability. We noted that FITC labeled peptides tend to deteriorate more quickly than the corresponding FAM conjugates.

Spectral properties: Abs/Em = 494/519 nm
Solvent system: DMF or DMSO
Appearance: Orange solid, Solvent System DMF or DMSO, used to prepare fluoresceinated bioconjugates
Storage Temperature: -20°C desiccated and protected from light
Shipping condition: RT

Formula: C₄₂H₂₄O₁₄
MW: 752.63 g/mol
Storage Temperature: Freezer

MDL Number: MFCD00151081
CAS Number: 72088-94-9

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