LigaFast Rapid DNA Ligation System, Promega

Supplier: Promega

LigaFast Rapid DNA Ligation System, Promega

LigaFast Rapid DNA Ligation System, Promega

M8221 M8225

PAM8221EA 132.96 USD

PAM8221 PAM8225

LigaFast Rapid DNA Ligation System, Promega

Nucleic Acid Reagents Cloning Kits and Ligation

T4 DNA Ligase and buffer combination for rapid (5-minute) ligations.

For Ligation and Cloning
Ligate DNA Inserts into Plasmid Vectors in 15 Minutes or Less at Room Temperature
DNA inserts with blunt ends or 5´ or 3´ overhangs can be used for ligation
No purification of ligated DNA needed prior to heat-shock transformation in E. coli
Ligations performed using the LigaFast™ System are comparable to standard overnight ligations

The LigaFast Rapid DNA Ligation System is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes). Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer. The LigaFast System is designed to eliminate any further purification prior to transformation of ligated DNA. The specially formulated 2X Rapid Ligation Buffer requires no additional ATP or Mg(2+) addition prior to use.

Storage: -30°C to -10°C

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