HaloTag Ligand Building Blocks are designed for use with HaloTag fusion proteins and can carry a variety of functionalities, including fluorescent labels, affinity tags and attachments to a solid phase.
- Enables any Reporter, Protein or Nucleic Acid with Specified Functional Groups to be used with the HaloTag(R) Protein
- Image live or fixed mammalian cells expressing HaloTag(R) protein or protein fusions
- Analyze post-translational modification
- Isolate proteins and protein complexes
- Cat.# P1681 and P6761 are discontinued.
The HaloTag Ligand Building Blocks can carry a variety of functionalities, including fluorescent labels, affinity tags and attachments to a solid phase. The covalent bond forms rapidly under general physiological conditions, is highly specific and essentially irreversible. The HaloTag Ligand Building Blocks allow researchers to apply the chloroalkane group that HaloTag protein reacts with to any compound or surface with a compatible chemical group, creating endless possible applications. The HaloTag Succinimidyl Ester (04) Ligand contains a reactive succinimidyl ester (SE) group connected to an alkyl chloride separated by three ethylene glycol repeats (04). The HaloTag Succinimidyl Ester (04) Ligand can be successfully conjugated to any reporter group, protein, or nucleic acid derivative containing an amine, forming stable amide bond linkages. The ligand with functional group can then be used with the HaloTag protein for any application of interest. The HaloTag Succinimidyl Ester (O2) Ligand contains a reactive succinimidyl ester (SE) group connected to an alkylchloride separated by an ethylene glycol repeat (O2). The HaloTag Succinimidyl Ester (O2) Ligand can be successfully conjugated to any reporter group, protein, or nucleic acid derivative containing an amine, forming stable amide bond linkages. The ligand with functional group can then be used with the HaloTag protein for any application of interest. The HaloTag Amine (04) Ligand contains a reactive amine group connected to an alkyl chloride, separated by an ethylene glycol repeat (04). The HaloTag Amine (04) Ligand can be successfully conjugated to any reporter group, protein, or nucleic acid derivative containing an activated carboxylic acid, sulfonyl halide or isocyanate. Examples of activated carboxylic acids are succinimidyl esters, STP esters, acid halides, and TFP esters. The ligand with functional group can then be used with the HaloTag protein for any application of interest. The HaloTag Amine (O2) Ligand contains a reactive amine group connected to an alkylchloride, separated by an ethylene glycol repeat (O2). The HaloTag Amine (O2) Ligand can be successfully conjugated to any reporter group, protein, or nucleic acid derivative containing an activated carboxylic acid, sulfonyl halide or isocyanate. Examples of activated carboxylic acids are succinimidyl esters, STP esters, acid halides and TFP esters. The ligand with functional group can then be used with the HaloTag protein for any application of interest. The HaloTag Iodoacetamide (04) Ligand contains a reactive iodoacetamide group connected an alkyl chloride separated by an ethylene glycol repeat (04). The HaloTag Iodoacetamide (04) Ligand has been designed to rapidly react with sulfhydryl-containing molecules, whether small organic compounds, peptides, or proteins. The ligand with functional group can then be used with the HaloTag protein for any application of interest. The HaloTag Iodoacetamide (O2) Ligand contains a reactive iodoacetamide group connected to an alkylchloride separated by an ethylene glycol repeat (O2). HaloTag Iodoacetamide (O2) Ligand has been designed to rapidly react with sulfhydryl-containing molecules, whether small organic compounds, peptides or proteins. The ligand with functional group can then be used with the HaloTag protein for any application of interest. The HaloTag Thiol (O4) Ligand contains a reactive sulfhydryl group connected to an alkyl chloride separated by three ethylene glycol repeats (04). The HaloTag Thiol (04) Ligand can be successfully conjugated to any reporter group, cross-linking reagent (bound or free), or nucleic acid derivative containing a number of different alkylating groups, forming stable thioether bonds. Commonly used reagents that rapidly react with sulfhydryls include iodo- or bromo-acetyls or benzyls, bromo- or chloro-mustards, maleimides, aziridines, acryloyl derivatives, and halide or sulfonate containing arenes (those bearing Electron Withdrawing Groups (EWGs) react most rapidly). The reactive ligand can be captured with HaloTag protein either before or after the thiol group is functionalized for any application of interest.
