Proteasome Purification Kit, Enzo Life Sciences

Supplier: Enzo Life Sciences
PW1075A-0001
76002-400EA 498 USD
76002-400
Proteasome Purification Kit, Enzo Life Sciences
Protein Purification Protein Purification Kits
High integrity kit for purification of proteasome complexes.

  • Isolation of wild type proteasome complexes from biological samples (cell lysates, tissue extracts, plasma, serum), confirmed by Western blot analysis
  • Analysis of captured proteasome complex proteins by proteomic methods, for example to determine complex composition and subunit post-translational modification
  • Investigation of variations in proteasome complexes in abnormal cell lines/tissues
  • Comparison of proteasome complexes isolated from biological samples associated with a particular disease/illness with samples from healthy controls

For the isolation and enrichment of proteasome complexes.

This kit provides a gentle and rapid means to purify proteasome complexes from biological samples using an agarose immobilized 20S subunit specific proteasome antibody, without the need for genetic modification of the expressed proteasome. Captured proteasomes are eluted under denaturing conditions prior to analysis by Western blotting or characterisation by proteomic methods.

Ordering information: Contains Proteasome purification matrix (BML-PW9005-0250), Control lysate (BML-SW8750-0100), Proteasome 20S β5, pAb (BML-PW8895-0010).
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