TNT SP6 High-Yield Wheat Germ Protein Expression System, based on an optimized wheat germ extract, is a single-tube, coupled transcription/translation system designed to express proteins in only two hours.
- Coupled Transcription/Translation for Soluble, Full-Length Protein in Only Two Hours
- Express soluble, full-length protein from plasmid or PCR-generated templates quickly
- Generate 10- to 20-fold more protein (10-100microg/ml) than with other cell-free systems
The TNT SP6 High-Yield Wheat Germ Protein Expression System, based on an optimized wheat germ extract, is a single-tube, coupled transcription/translation system designed to express proteins in only two hours. Protein synthesized, in the range of 10-100ug/ml, can be used in multiple proteomic-based applications, as well as in high-throughput analysis. All components necessary for transcription/translation are provided in the extract, with the exception of the plasmid DNA or PCR template. Optional protein-labeling reagents must also be supplied by the user. For custom wheat germ extract (depleted amino acids), order Cat.# X808X (see Products, Available Separately).
With the exception of the plasmid DNA or PCR template, all components necessary for transcription/translation are provided in the extract. Optional protein-labeling reagents must also be supplied by the user.