Bovine Fibrinogen (from Plasma), MP Biomedicals
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
Background Information
Fibrinogen is a blood protein that is involved in clotting and is converted to fibrin by thrombin. Fibrinogen has an approximate molecular weight of 340 kDa. It consists of three non-identical pairs of disulfidebonded chains. The α-chain has an approximate molecular weight of 63.5 kDa, the β-chain 56 kDa, and the γ-chain 47 kDa. At the amino termini, the three chains are connected in a dimeric disulfide knot (DSK). A second DSK occurs later in the molecule. Fibrinogen has approximately 4% carbohydrate content.
- Gene ID:Cow- FGA(522039), FGB(510522), FGG(280792)
- Protein:80% w/w, balance excipients
- Clottable Protein: > 90%
- Excipients : Trisodium Citrate, NaCl, Tween 80
- Moisture: 1.00%
Applications
Bovine fibrinogen has been used in studies of haemostatic therapy in surgical and massive trauma patients. These studies have shown that fibrinogen may prove to be more superior in stopping blood loss when compared to using fresh frozen plasma (FFP). It can be used for preparation of fibrin plates for analysis of fibrinolytic enzymes, a substrate for clotting assays, and study of fibrinogen degradation products.
Biochem/physiol Actions
Fibrinogen is an acute phase protein that is part of the coagulation cascade of proteins. The end result of the cascade is the production of thrombin that converts fibrinogen to fibrin. Thrombin rapidly proteolyzes fibrinogen, releasing fibrinopeptide A. The loss of this small peptide is not sufficient to make the resulting fibrin molecule insoluble, but it tends to form complexes with adjacent fibrin and fibrinogen molecules. Thrombin then cleaves a second peptide, fibrinopeptide B, from fibrin and the fibrin monomers formed then polymerize spontaneously to form an insoluble gel. The polymerized fibrin is held together by noncovalent and electrostatic forces and stabilized by the transamidating enzyme, factor XIIIa, that is produced by the action of thrombin on factor XIII. The insoluble fibrin aggregates (clots) and aggregated platelets then block the damaged blood vessel and prevent further bleeding. The amount of fibrinogen in the plasma can serve as a nonspecific indicator of whether or not an inflammatory process is present in the body. Fibrinogen from any mammalian source will be cleaved by thrombin from any mammalian source.
Recommended Use
Reconstitution: Fibrinogen has varied solubility under different conditions ; in particular, ionic strength can have a significant influence. The % protein reported above was obtained as follows: Solubilization buffer: 0.9% NaCl; Nominal powder concentration: 10-20 mg/mL; Solubilization temperature and time: RT for 30 min; Frequent gentle mixing followed by centrifugation to clarify, Clarified solution will have 90% clottability. The reconstitution parameters above are provided as a guideline for using the product. The parameters may be modified as desired and individual results may vary.
Since fibrinogen is very similar among species, correspondingly similar characteristics would be expected for bovine, cat, dog, guinea pig, human, sheep, mouse, and rat fibrinogens. In general, fibrinogen from any mammalian source will cross-react with thrombin from any mammalian source. When any mammalian thrombin is injected into a different mammal, clotting will occur.
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