Pierce™ Recombinant Protein L (from E. coli), Biotin

Supplier: Pierce Chemical
Pierce
29997 29997
PI29997EA 377.97 USD
PI29997 CAPI29997
Pierce™ Recombinant Protein L (from E. coli), Biotin
Proteins and Peptides
Pierce™ Biotinylated Recombinant Protein L can be used to probe and detect mouse and human antibodies containing kappa light chains in Western blotting, ELISA, IHC and other immunoassay protocols.

  • Contains four immunoglobulin binding domains per protein
  • Binds members of all classes of Ig that contain kappa light chains
  • Binds single chain variable fragments (scFv) and Fab fragments that contain kappa light chains

Protein L is an immunoglobulin-binding protein that was originally derived from the bacteria Peptostreptococcus magnus, but is now produced recombinantly. Protein L has the unique ability to bind through kappa light chain interactions without interfering with an antibody's antigen-binding site. This gives Protein L the ability to bind a wider range of Ig classes and subclasses than other antibody-binding proteins. Protein L will bind to all classes of Ig (IgG, IgM, IgA, IgE and IgD). Protein L will also bind single chain variable fragments (scFv) and Fab fragments. Protein L binds kappa I, III, and IV in human and kappa I on mouse.

Form: salt-free powder
A280 of 0.1% solution:

0.80
Isoelectric point (pI): 4.8
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