Thermo Scientific Pierce Protein L Magnetic Beads are high-capacity and high-throughput affinity particles for antibody purification using manual or robotic magnetic separators.
- Immobilized Protein L is Ideal for Selective Purification of Human and Mouse Antibodies that have kappa Light Chains
- Low Non-Specific Binding of Stable, Pre-Blocked Beads Provide Clean Purification of Antibody
- Beads are Compatible with Manual and Automated Workflows Making it Versatile
Recombinant Protein L, covalently attached to a blocked magnetic bead surface, selectively binds mouse and human antibodies through kappa light chains
Thermo Scientific Pierce Protein L Magnetic Beads are typically used for purifying mouse and human antibodies containing kappa light chains from serum, cell culture supernatant or ascites. Protein L can bind a broader range of Ig classes than Protein A or Protein G, including IgG, IgM, IgA, IgE and IgD. Protein L binds strongly to human (kappa I, III and IV only), mouse (kappa I only), rat and pig immunoglobulins. It binds weakly to rabbit immunoglobulins and does not bind bovine, goat or sheep immunoglobulins. Single chain variable fragments (scFv) and Fab fragments also bind to Protein L. The protocol for the Pierce Protein L beads has been optimized to allow for high recovery and high purity of isolated antibodies.
The beads are commonly used to purify monoclonal antibodies in cell culture supernatants supplemented with bovine serum as Protein L does not bind bovine IgG. The Pierce Protein L Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms such as the Thermo Scientific KingFisher Instruments for high-throughput workflows.
Ordering information: Upon receipt store at 4?C. Product shipped with an ice pack.
Caution: For Research Use Only. Not for use in diagnostic procedures.
