Pierce™ Maleimide Activated Plates, Thermo Scientific

Supplier: Thermo Scientific
Pierce
15153 15153 15152 15152 15150 15150
PI15150PK 254.58 USD
PI15150 CAPI15153 CAPI15150 CAPI15152 PI15153 PI15152
Pierce™ Maleimide Activated Plates, Thermo Scientific
Microplates Immunology Microplates and Strips
Pierce™ Maleimide activated plates are ideal for binding sulfhydryl-containing molecules that are difficult to coat onto polystyrene plates, such as peptides that contain a terminal cysteine. Available in clear for colorimetric assays, white for chemiluminescent assays and black for fluorescent assays.

  • Pre-blocked to reduce nonspecific binding
  • Easy (spontaneous) immobilization of peptides derivatized with a terminal cysteine and proteins with free sulfhydryl
  • Binding Capacity: 100 to 150 pmol of a sulfhydryl-containing peptide (307 Da) per well
  • Activation Level: 100 µL

Our coated plates are an especially useful tool for assessing specific anti-hapten antibody titers during antibody production.

Maleimides react with free sulfhydryl group(s) at pH of 6.5-7.5, forming stable thioether linkages. Reaction with amines becomes significant at pH> 7.5. Some sulfhydryl-containing peptides and proteins may oxidize in solution and form disulfide bonds, which cannot react with maleimides. Disulfide bonds can be reduced to produce free sulfhydryls.

Caution: For Research Use Only. Not for use in diagnostic procedures.
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