NEB® PCR Cloning Kit, New England Biolabs

Supplier: New England Biolabs

NEB PCR Cloning Kit - 20 rxns

NEB PCR Cloning Kit - 20 rxns

E1202S E1202S

102877-564EA 324 USD

CA102877-564 102877-564

NEB® PCR Cloning Kit, New England Biolabs

Nucleic Acid Reagents Cloning Kits and Ligation

This PCR Cloning Kit contains an optimized 2X Cloning Master Mix with a proprietary ligation enhancer and a linearized vector that uses a novel mechanism for background colony suppression to give a low background.

It allows simple and quick cloning of any PCR amplicon, whether the amplification reactions are performed with proofreading DNA polymerases, such as Q5® or Phusion® which produce blunt ends; or nonproofreading DNA polymerases, such as Taq or Taq mixes (OneTaq®, LongAmp™ Taq) which produce single base overhangs. This is possible due to “invisible” end polishing components in the master mix that are active during the ligation step. The kit also allows direct cloning from amplification reactions without purification, and works well whether or not the primers used in the PCR possess 5´-phosphate groups.

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