Thermostable 5' AppDNA/RNA Ligase, New England Biolabs

Supplier: New England Biolabs
Ratings: (No Reviews)

Total Ratings: 0
Avg. Ratings: 0.0 out of 5

M0319L M0319L M0319S M0319S
102715-906EA 81 USD
CA102715-904 102715-906 102715-904 CA102715-906
Thermostable 5' AppDNA/RNA Ligase, New England Biolabs
Nucleic Acid Reagents Cloning Kits and Ligation
Thermostable 5´ App DNA/RNA Ligase is a point mutant of catalytic lysine of RNA ligase from Methanobacterium thermoautotrophicum

This enzyme is ATP independent. It requires a 5´ pre-adenylated linker for ligation to the 3´-OH end of either RNA or single stranded DNA (ssDNA). The enzyme is also active in ligation of RNA with 2´-O-methylated 3´ end to 5´-adenylated linkers. The optimal temperature for ligation reaction is 60–65°C. The mutant ligase is unable to adenylate the 5´-phosphate of RNA or ssDNA, which reduces the formation of undesired ligation products (concatemers and circles).
Order Now

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR