QUICK BLUNTING™ and QUICK LIGATION™ Kits, New England Biolabs

Supplier: New England Biolabs
E0542S E0542S
101417-934EA 182 USD
CA101417-934 101417-934
QUICK BLUNTING™ and QUICK LIGATION™ Kits, New England Biolabs
Nucleic Acid Reagents Cloning Kits and Ligation
NEB offers the Quick Blunting™ and Quick Ligation™ Kits together as a bundle at a value price.

The Quick Blunting™ Kit is used to convert DNA with incompatible 5´or 3´overhangs to 5´phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors. DNA is blunted using T4 DNA polymerase which has both 3´→ 5´ exonuclease activity and 5´→ 3´ polymerase activity. T4 Polynucleotide Kinase is included in the enzyme mix for phosphorylation of the 5´ends of blunt-ended DNA for subsequent ligation into a cloning vector. This kit is optimized for blunting up to 5 µg of DNA in a single reaction. The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature (25°C).
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