For use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins.
Lyso-ID® Red Lysosomal Detection Kit (GFP-Certified®) is an ideal choice for fluorescence co-localization imaging with GFP-tagged proteins, providing spectrally pure red signal
The Lyso-ID® Red Lysosomal Detection Kit (GFP-Certified®)contains a proprietary acidic organelle-selective dye suitable for staining live cells. The dye is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes, as well as High Content Screening (HCS) platforms. This kit is specifically designed for use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins (BFPs, CFPs, YFPs).
The Lyso-ID® Red dye has been validated for utility in live cell imaging applications, showing an appropriate response to treatment with well characterized lysosomal perturbation agents. A lysosome perturbation agent, chloroquine, is provided as a positive control for monitoring changes in lysosome number and volume. A nuclear counterstain (Hoechst 33342) is provided to highlight this organelle as well.
By contrast, many red-emitting lysosomal probes photoconvert to green fluorescent species or display metachromatic artifacts wherein emission occurs both in the red and green regions of the spectrum, leading to spurious results in GFP co-localization experiments.
Delivery information: Kit contains Lyso-ID® Red Detection Reagent, 50 µL, Hoechst 33342 Nuclear Staining, 50 µL, Chloroquine Control, 7.5 µmol and10X Assay Buffer, 15 mL.
