A Fluor de Lys® fluorescent assay system
- Useful for inhibitor screening or characterizing enzyme kinetics
- Includes optimal substrate selected from a panel of acetylated sites in p53 and histones
- Supplied with enough recombinant enzyme for 96 assays (1 x 96-well plate)
The HDAC6 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC6 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions. The Fluor de Lys® HDAC6 assay is based on the Fluor de Lys® Substrate and Fluor de Lys® Developer combination. The assay procedure has two steps. First, the Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with HDAC6. Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys®Developer produces a fluorophore.
Ordering information: Quantity: 96 assays
Contents:
HDAC6 (Histone Deacetylase 6) (human, recombinant) (Prod. No. BML-SE508)
(50 µg; 10mM TRIS, pH 7.5, 100mM sodium chloride, 3mM magnesium chloride, 10% glycerol)
Storage: -70°C, avoid freeze/thaw cycles!
FLUOR DE LYS® SIRT1, Deacetylase Substrate (Prod. No. BML-KI177)
(100µl; 5mM solution in 50mM TRIS/Cl, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride)
Storage: -70°C
FLUOR DE LYS® Developer II Concentrate (5x) (Prod. No. BML-KI176)
(5 x 250 µl; 5x Stock Solution; Dilute in Assay Buffer before use
Storage: -70°C
Trichostatin A (HDAC Inhibitor) (Prod. No. BML-GR-309-9090)
(100 µl; 0.2mM in DMSO)
Storage: -70°C
FLUOR DE LYS® Deacetylated Standard (Prod. No. BML-KI142)
(30 µl; 10mM in DMSO)
Storage: -70°C
HDAC Assay Buffer II (Prod. No. BML-KI422)
(20ml; 50mM TRIS/Cl, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride, 1mg/ml bovine serum albumin)
Storage: -70°C
HDAC Assay Buffer (Prod. No. BML-KI143)
(20ml; 50mM TRIS/Cl, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride)
Storage: -70°C
1/2 volume microplate (Prod. No. BML-KI101)
Storage: Room temperature
1/2 volume white NBS microplate (Prod. No. BML-KI571)
Storage: Room temperature
Delivery information: Kit Contains, HDAC6 (Histone Deacetylase 6) (human, recombinant) (50 µg; 10mM TRIS, pH 7.5, 100mM sodium chloride, 3mM magnesium chloride, 10% glycerol), Fluor-de-Lys® SIRT1, Deacetylase Substrate (100µL; 5mM solution in 50mM TRIS/Cl, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride), Fluor-de-Lys® Developer II Concentrate (5x) (5 x 250 µL; 5x Stock Solution; Dilute in Assay Buffer before use), Trichostatin A (HDAC Inhibitor) (100 µL; 0.2mM in DMSO), Fluor-de-Lys® Deacetylated Standard (30 µL; 10mM in DMSO), HDAC Assay Buffer II (20mL; 50mM TRIS/Cl, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride, 1mg/ml bovine serum albumin), HDAC Assay Buffer (20mL; 50mM TRIS/Cl, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride), 1/2 volume microplate and 1/2 volume white NBS microplate.
