You Searched For: Enzyme Assays

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.


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Supplier: Promega Corporation
Description: Recombinant human DDR2 (467-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. DDR2 is a member of a novel subclass of RTKs containing a distinct extracellular region encompassing a factor VIII-like domain.

SDS

Catalog Number: (PAV5095)
Supplier: Promega Corporation
Description: Recombinant full-length human PKCbeta I was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKCβI is a member of the PKC family (phospholipid-dependent serine/threonine kinase) and is highly related to PKCβ II.

SDS


Supplier: Promega Corporation
Description: Recombinant full-length human CHK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CHK2 is rapidly phosphorylated and activated in response to replication blocks and DNA damage.

SDS

Supplier: Promega Corporation
Description: Recombinant full-length human NUAK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NUAK2 or SNF1/AMP kinase-related kinase (SNARK) is a member of the NUAK family of SNF1-like kinase 2.

SDS

Supplier: Promega Corporation
Description: The ROS-Glo H2O2 Assay is a homogeneous, fast and sensitive bioluminescent assay that measures the level of hydrogen peroxide (H2O2), a reactive oxygen species (ROS), directly in cell culture or in defined enzyme reactions.
Supplier: Promega Corporation
Description: The Proteasome-Glo Cell-Based Assays are homogeneous, luminescent assays that measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome complex in cultured cells.

Supplier: Promega Corporation
Description: Recombinant human TGF beta R2 (190-end) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. TGF beta R2 is a member of the TGF beta receptor subfamily and is a ser/thr protein kinase.

SDS

Supplier: Promega Corporation
Description: Recombinant full-length human JNK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. JNK3 is a member of the c-Jun N-terminal kinases (JNKs), which are part of the mitogen-activated protein (MAP) kinase family.

SDS

Catalog Number: (PAV4085)
Supplier: Promega Corporation
Description: RIPK2 (RIP2; RICK) is a death domain-containing protein kinase encoding a predicted 540-amino acid protein that contains an N-terminal serine/threonine kinase catalytic domain and a C-terminal caspase activation and recruitment domain.


Catalog Number: (PAV4075)
Supplier: Promega Corporation
Description: Recombinant human MYO3 beta (1-326) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MYO3 beta is a member of the Class III myosins that are actin-dependent motor proteins containing an amino-terminal kinase domain.


Catalog Number: (PAV4069)
Supplier: Promega Corporation
Description: Full-length recombinant human IKK alpha was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. IKK alpha is a serine/threonine protein kinase that phosphorylates the I-kappa-B protein.


Catalog Number: (PAV4077)
Supplier: Promega Corporation
Description: Recombinant human NIK (325-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NIK is a mitogen-activated protein kinase kinase kinase 14 (MAP3K14), which binds to TRAF2 and stimulates NF-kappaB activity.


Supplier: Promega Corporation
Description: Recombinant full-length human PIM2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PIM2 is a serine threonine kinase that is present in all tissues.

Catalog Number: (PAV4031)
Supplier: Promega Corporation
Description: Recombinant full-length human p70S6Kb was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. p70S6Kb is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases and is activated by mitogenic stimuli.


Supplier: Promega Corporation
Description: Recombinant full-length human MARK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MARK1 is a member of the MARK family and is a serine/threonine-protein kinase that plays a key role in signal transduction.

Catalog Number: (PAV4041)
Supplier: Promega Corporation
Description: Recombinant full-length human PKC theta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Protein Kinase C theta (PKC theta) is an important component in the intracellular signaling cascade.


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