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You Searched For: Enzyme Assays

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.


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Product Image-PAV4476

MLK2 Kinase Enzyme System, Promega

Supplier: Promega Corporation
Description: Recombinant human MLK2 (1-446) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MLK2 or mixed-lineage kinase 2 is a member of the serine/threonine kinase family, which activates MAPK8/JNK1 and MKK4/SEK1.

SDS

Product Image-PAV4478

PAK1/CDC42 Kinase Enzyme System, Promega

Supplier: Promega Corporation
Description: Recombinant full-length human PAK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag and combined with CDC42 at a ratio of 1:4 (PAK1:CDC42) in vitro. PAK1 is a member of the p21-activated kinases (PAKs).

SDS

Product Image-PAV4028

MARK1 Kinase Enzyme System, Promega

Supplier: Promega Corporation
Description: Recombinant full-length human MARK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MARK1 is a member of the MARK family and is a serine/threonine-protein kinase that plays a key role in signal transduction.

SDS

Product Image-PAV4034

PIM2 Kinase Enzyme System, Promega

Supplier: Promega Corporation
Description: Recombinant full-length human PIM2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PIM2 is a serine threonine kinase that is present in all tissues.

SDS

Product Image-PAV4244

ZAK Kinase Enzyme System, Promega

Supplier: Promega Corporation
Description: Recombinant full-length human ZAK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ZAK is a member of the MAPKKK family of signal transduction molecules and mediates gamma radiation signaling leading to cell cycle arrest.

SDS

Product Image-PAV4085

ADP-Glo Kinase Assay + RIPK2 Kinase Enzyme System, 1 each, Promega

Catalog Number: (PAV4085)
Supplier: Promega Corporation
Description: RIPK2 (RIP2; RICK) is a death domain-containing protein kinase encoding a predicted 540-amino acid protein that contains an N-terminal serine/threonine kinase catalytic domain and a C-terminal caspase activation and recruitment domain.
Product Image-PAV4075

ADP-Glo Kinase Assay + MYO3 beta Kinase Enzyme System, 1 each, Promega

Catalog Number: (PAV4075)
Supplier: Promega Corporation
Description: Recombinant human MYO3 beta (1-326) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MYO3 beta is a member of the Class III myosins that are actin-dependent motor proteins containing an amino-terminal kinase domain.
Product Image-PAV4069

ADP-Glo Kinase Assay + IKK alpha Kinase Enzyme System, 1 each, Promega

Catalog Number: (PAV4069)
Supplier: Promega Corporation
Description: Full-length recombinant human IKK alpha was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. IKK alpha is a serine/threonine protein kinase that phosphorylates the I-kappa-B protein.
Product Image-PAV4077

ADP-Glo Kinase Assay + NIK Kinase Enzyme System, 1 each, Promega

Catalog Number: (PAV4077)
Supplier: Promega Corporation
Description: Recombinant human NIK (325-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NIK is a mitogen-activated protein kinase kinase kinase 14 (MAP3K14), which binds to TRAF2 and stimulates NF-kappaB activity.
Product Image-PAV4479

ADP-Glo Kinase Assay + PAK1/CDC42 Kinase Enzyme System, 1 each, Promega

Catalog Number: (PAV4479)
Supplier: Promega Corporation
Description: Recombinant full-length human PAK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag and combined with CDC42 at a ratio of 1:4 (PAK1:CDC42) in vitro. PAK1 is a member of the p21-activated kinases (PAKs).
Product Image-PAV4099

ADP-Glo Kinase Assay + HPK1 Kinase Enzyme System, 1 each, Promega

Catalog Number: (PAV4099)
Supplier: Promega Corporation
Description: Recombinant human HPK1 (1-346) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. HPK1 or Hematopoietic progenitor kinase 1 is a hematopoietic cell-restricted member of the Ste20 serine/threonine kinase super family.
Product Image-PAV4165

ADP-Glo Kinase Assay + HIPK3 Kinase Enzyme System, 1 each, Promega

Catalog Number: (PAV4165)
Supplier: Promega Corporation
Description: Recombinant human HIPK3 (163-562) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. HIPK3 (homeodomain interacting protein kinase 3) is a member of the HIPK family and is involved in apoptosis.
Product Image-PAV4485

ADP-Glo Kinase Assay + CK1 alpha 1 Kinase Enzyme System, 1 each, Promega

Catalog Number: (PAV4485)
Supplier: Promega Corporation
Description: Full-length recombinant human CK1 alpha 1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CK1 alpha 1 is a member of the CK1 family of serine/threonine protein kinases, which play an important role in diverse cell processes.
Product Image-PAV4477

ADP-Glo Kinase Assay + MLK2 Kinase Enzyme System, 1 each, Promega

Catalog Number: (PAV4477)
Supplier: Promega Corporation
Description: Recombinant human MLK2 (1-446) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MLK2 or mixed-lineage kinase 2 is a member of the serine/threonine kinase family, which activates MAPK8/JNK1 and MKK4/SEK1.
Product Image-PAV4151

ADP-Glo Kinase Assay + MELK Kinase Enzyme System, 1 each, Promega

Catalog Number: (PAV4151)
Supplier: Promega Corporation
Description: Recombinant human MELK (1-340) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MELK or maternal embryonic leucine zipper kinase is a member of the Snf1/AMPK kinase family.
Product Image-PAV4241

ADP-Glo Kinase Assay + PASK Kinase Enzyme System, 1 each, Promega

Catalog Number: (PAV4241)
Supplier: Promega Corporation
Description: Recombinant human PASK (981-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PASK or PAS domain containing serine/threonine kinase regulates the function of many intracellular signaling pathways involved in stress.
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