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You Searched For: Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Product Image-PAM1801

T4 DNA Ligase, Promega

Supplier: Promega Corporation
Description: For ligation of DNA ends, mutagenesis and cDNA cloning.

Certificates

Product Image-103218-906

NEBuilder HiFi DNA Assembly Bundle for Large Fragments, New England Biolabs

Catalog Number: (103218-906)
Supplier: New England Biolabs (NEB)
Description: NEBuilder HiFi DNA Assembly was developed to improve the efficiency and accuracy of DNA assembly

Small Business Enterprise


Product Image-PAP9801

Bacterial Strain JM109(DE3), Glycerol Stock, Promega

Catalog Number: (PAP9801)
Supplier: Promega Corporation
Description: Used for high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter, if the sequence contains a ribosomal binding site. No blue/white selection.

Certificates


Product Image-101228-188

T4 RNA Ligase 1 (ssRNA Ligase), New England Biolabs

Supplier: New England Biolabs (NEB)
Description: T4 RNA Ligase 1 catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid donor to a 3' hydroxyl-terminated nucleic acid acceptor through the formation of a 3'→5' phosphodiester bond, with hydrolysis of ATP to AMP and PPi

Small Business Enterprise

Product Image-101228-194

E. coli DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA containing cohesive ends.

Small Business Enterprise

Product Image-PAC8640

Flexi System, Entry/Transfer, 5 entry and 20 transfer reactions, Promega

Catalog Number: (PAC8640)
Supplier: Promega Corporation
Description: The Flexi Vector System provides a rapid, efficient, high-fidelity way to transfer protein-coding regions without the need to resequence.
Product Image-102715-944

Instant Sticky-end Ligase Master Mix, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Instant Sticky-end Ligase Master Mix is a ready-to-use 2X solution of T4 DNA ligase and a proprietary ligation enhancer in an optimized reaction buffer.

Small Business Enterprise

Product Image-102877-808

NEBUILDER HiFi DNA Assembly® Cloning Kit, New England Biolabs

Catalog Number: (102877-808)
Supplier: New England Biolabs (NEB)
Description: The NEBuilder HiFi DNA Assembly Cloning Kit combines the power of the NEBuilder HiFi DNA Assembly Master Mix with NEB 5-alpha Competent E

Small Business Enterprise


Product Image-101228-182

T4 DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.

Certificates Small Business Enterprise

Product Image-102969-106

NEBNext Ultra II Ligation Module, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: The NEBNext Ultra II Ligation Module has been optimized to efficiently ligate DNA adaptors compatible with Illumina® sequencing to end-repaired, dA-tailed DNA fragments

Small Business Enterprise

Image Unavailable-PAG6050

HaloTag Cloning Starter System, 1 each, Promega

Catalog Number: (PAG6050)
Supplier: Promega Corporation
Description: HaloTag Cloning Starter Kit: designed to use Flexi Cloning System to clone protein coding sequences into mammalian HaloTag expression vectors; clone and transfer gene sequences into C- or N-terminus of HaloTag Flexi CMV Deletion Vectors.
Product Image-103307-256

RtcB Ligase

Catalog Number: (103307-256)
Supplier: New England Biolabs (NEB)
Description: RtcB Ligase is expressed as a His-tagged fusion in <i>E. coli</i>.

Small Business Enterprise


Product Image-101417-962

T4 RNA Ligase 2 Truncated, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: T4 RNA Ligase 2, truncated (T4 Rnl2 truncated) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of RNA.

Small Business Enterprise

Product Image-PAP9751

Bacterial Strain JM109, Promega

Catalog Number: (PAP9751)
Supplier: Promega Corporation
Description: Useful host for transformation of pGEM Vectors and production of ssDNA from M18 or phagemid vectors. JM109 is recA- and lacks the E. coli K restriction system. Can be used for blue/white screening.

Certificates


Product Image-PAM1794

T4 DNA Ligase, Promega

Catalog Number: (PAM1794)
Supplier: Promega Corporation
Description: For ligation of DNA ends, mutagenesis and cDNA cloning

Certificates


Product Image-101640-956

T4 RNA Ligase 2 Truncated, K227Q, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: T4 RNA Ligase 2, truncated K227Q (T4 Rnl2tr K227Q) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ OH end of RNA.

Small Business Enterprise

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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