PneumaCult™ Apical-Out Airway Organoid Medium

Supplier: STEMCELL Technologies

100-0620
MSPP-100-0620EA 1243.08 USD
MSPP-100-0620
PneumaCult™ Apical-Out Airway Organoid Medium
Cell Culture Media

Serum- and BPE-free medium for differentiation of human primary bronchial epithelial cells or human airway epithelial cells to mature apical-out airway organoids.


Generate apical-out airway organoids in 15 days from human bronchial epithelial cells (HBECs) or human airway epithelial cells (HAECs) with a simple and scalable, Matrigel®-free protocol. These organoids provide access to the apical side of the airway epithelium and can be used to perform infectious disease modeling or high-throughput drug screening in an in vitro, three-dimensional (3D) model system. PneumaCult™ Apical-Out Airway Organoid Medium allows growth of polarized airway organoids with outward-facing, differentiated ciliated cells in AggreWell™400 24-well plates - without the use of cell culture inserts. To maximize experimental reproducibility, this medium is formulated without serum or BPE. For a complete apical-out airway organoid culture workflow, expand HBECs or HAECs in PneumaCult™-Ex Plus Medium prior to initiating organoid culture with PneumaCult™ Apical-Out Airway Organoid Medium.


Caution: Product restrictions: (i) Customer shall not make products or any portion of them in any way, shape or form, including as a component of another product available for the purpose of further resale or alter or remove the Product label and the Supplier’s mark of origin without express written permission of Supplier and (ii) Customer is familiar with Supplier’s restrictions on the use of the products and shall use the products in compliance with such restrictions and the applicable Supplier Product Information sheet (collectively, the 'Product Restrictions').

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