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DNA transfection reagent - jetPRIME®

jetPRIME® is a powerful and versatile DNA and/or siRNA transfection reagent designed to ensure high nucleic acid transfection efficiency and excellent gene silencing in a variety of adherent cells.

  • High DNA transfection efficiency
  • Low amounts of nucleic acid
  • Superior cell viability
  • ONE reagent for DNA and/or siRNA transfections
  • Easy to use
  • Cost-effective

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Description

jetPRIME® is a powerful and versatile DNA and siRNA transfection reagent for day-to-day experiments that leads to efficient and reliable scientific results. jetPRIME® ensures high DNA transfection efficiency and excellent gene silencing in a variety of adherent cells. jetPRIME® is also ideal for DNA/siRNA co-transfection or co-delivery of several plasmids.

Comparative transfection efficiency of jetPRIME® versus its main competitor. Transfection efficiency was assessed by FACS analysis in various cell lines 24 h after transfection in 24-well plates. Conditions used were according to the manufacturer's recommendation for competitors L2K and 0.5 µg plasmid + 1 µl reagent per well for jetPRIME ®.

Furthermore, jetPRIME® transfection reagent is very gentle on cells since it requires low amounts of reagent and nucleic acid during transfection.

Using less DNA minimizes adverse cytotoxic effects triggered by transfection. Hence, jetPRIME® is the reagent of choice for high transfection efficiency with excellent cell viability.

Cell viability 24h after transfection. Phase contrast microscopy of HeLa cells 24h after transfections performed according to the manufacturer's recommendations for each reagent.

jetPRIME® is extermely easy to use and is provided with its own buffer.

Examples of citations

Charrier, C., Joshi, K., Coutinho-Budd, J., Kim, J. E., Lambert, N., de Marchena, J., Jin, W. L., Vanderhaeghen, P., Ghosh, A., Sassa, T., Polleux, F. (2012).Inhibition of SRGAP2 function by its human-specific paralogs induces neoteny during spine maturation., Cell 149, 923-3.

Ichim, G., Genevois, A. L., Menard, M., Yu, L. Y., Coelho-Aguiar, J. M., Llambi, F., Jarrosson-Wuilleme, L., Lefebvre, J., Tulasne, D., Dupin, E., Le Douarin, N., Arumae, U., Tauszig-Delamasure, S., Mehlen, P. (2013). The Dependence Receptor TrkC Triggers Mitochondria-Dependent Apoptosis upon Cobra-1 Recruitment., Mol Cell 51, 632-4.

Shi, J., Zhao, Y., Wang, Y., Gao, W., Ding, J., Li, P., Hu, L., Shao, F. (2014). Inflammatory caspases are innate immune receptors for intracellular LPS., Nature 514, 187-9.

Thomas, M. G., Luchelli, L., Pascual, M., Gottifredi, V., Boccaccio, G. L. (2012). A monoclonal antibody against p53 cross-reacts with processing bodies., PLoS ONE 7, e3644.

Testimonials

“Very good transfection efficiency, better value and ease of use”
Sean H. MD Anderson Cancer Center, USAg

“jetPRIME has low toxicity and with less amount of DNA and the transfection reagent comparable expression can be achieved. I would prefer using jetPRIME over other reagents for the above mentioned reasons.”
Vijayalakshmi A, Baylor College of Medicine (BCM), USA

Product selection

jetMESSENGER™ mRNA transfection reagent

jetMESSENGER enables transfection in hard to transfect cells using mRNA. Take your gene expression to the next level by switching to mRNA!

INTERFERin® siRNA/miRNA transfection reagent

INTERFERin provides high silencing efficiency at low concentration of siRNA/miRNA that enables to avoid off-target effects. Its gentle mode of action ensure more robust data and excellent cell viability,

in vivo-jetPEI® for in vivo nucleic acid delivery

in vivo-jetPEI is a powerful polymer based reagent used to deliver any nucleic acid to any animal model. in vivo-jetPEI has already been used to target a wide range of organs using various administration routes.

jetCRISPR™ RNP transfection reagent

jetCRISPR™ is an innovative reagent especially designed to deliver RNP in a CRISPR/Cas9 experiment. Use the leading technology for CRISPR/Cas9 RNP delivery!