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Product Image-77393-238

PureCube Maleimide Activated MagBeads

Supplier: CUBE BIOTECH
Description: PureCube Maleimide MagBeads are your best option to couple biomolecules that carry thiol (SH) groups.

Product Image-77393-360

PureCube Carboxy MagBeads

Supplier: CUBE BIOTECH
Description: PureCube amine carboxy agarose MagBeads are your best option to couple biomolecules that carry free-standing Amine (NH₂) groups.

Product Image-77393-200

PureCube Epoxy Activated MagBeads

Supplier: CUBE BIOTECH
Description: PureCube epoxy activated MagBeads are your best option to couple biomolecules that carry free-standing amine (NH₂) and thiol (SH) groups.

Image Unavailable-77392-538

AASTY 6-50

Supplier: CUBE BIOTECH
Description: AASTYs (Acrylic acid-co-styrenes) - like AASTY 6-50 - are highly-alternating copolymers, well-suited for the generation of native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 6-50 gets its name from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general lighter AASTYs, like 6-50 tend to be more aggressive, while heavier AASTYs, such as 11-45 show higher thermodynamic stability.

The exact composition of AASTY copolymers shows different extraction efficiency, depending on the lipid composition of the lipid bilayers being formulated into nanodiscs. As AASTY is made using controlled radical polymerization techniques, the dispersity of polymer molecular weight distribution is low, and the molecular weights are controlled. This means that excess AASTY copolymer can be removed by dialysis after nanodisc formation. Based on previous findings on SMA, it is the expectation that AASTY of different molecular weights will display different rates of nanodisc formation, extraction efficacy, and stability of resulting nanodiscs.

Every membrane protein solubilization needs to undergo a screening process before. The characteristic phospholipid environment surrounding the different membrane proteins in question performs differently well with each polymer. To support you in this process we offer a handy Screening Kit for AASTYs to test them all. Additionally, we recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022

Product Image-77393-032

PureCube Compact Cartridge NTA

Supplier: CUBE BIOTECH
Description: The PureCube NTA (nitrilotriacetic acid) Compact Cartridge was developed for IMAC-based protein purification and protein enrichment methods.

Product Image-77393-220

PureCube Amine Activated Agarose

Supplier: CUBE BIOTECH
Description: PureCube amine activated agarose resin is your best option to couple biomolecules carrying free-standing carboxy (COOH) groups.

Image Unavailable-77392-650

HiYield-T7 Cell-free E. coli Lysate

Supplier: CUBE BIOTECH
Description: Our <i>E. coli</i> cell-free lysates are the isolated protein purification machinery of bacterial cells. They create a high-yielding and adjustable cell-free protein expression system.

Product Image-77393-292

PureCube NTA XL Agarose

Supplier: CUBE BIOTECH
Description: PureCube NTA XL (nitrilotriacetic acid) agarose resin was developed for IMAC-based protein purification and protein enrichment methods.

Product Image-77392-464

FPLC Column/Cartridge Assembly Press

Supplier: CUBE BIOTECH
Description: The FPLC Cartridge assembly press is an efficient tool that simplifies the packaging process for FPLC columns.

Product Image-77392-880

Aequorea victoria Recombinant eGFP Protein, His-Tag (from E. coli)

Supplier: CUBE BIOTECH
Description: Enhanced GFP (eGFP) is a modified version of GFP that exhibits even stronger fluorescence for easier detection.

Product Image-77392-548

AASTY 11-50

Supplier: CUBE BIOTECH
Description: AASTYs (Acrylic acid-co-styrenes) - like AASTY 11-50 - are highly-alternating copolymers, well-suited for the generation of native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 11-50 gets its name from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general lighter AASTYs, like 6-45 tend to be more aggressive, while heavier AASTYs, such as 11-50 show higher thermodynamic stability.

Every membrane protein solubilization needs to undergo a screening process in before. The characteristic phospholipid environment surrounding the different membrane proteins in question performs differently well with each polymer. To support you in this process, we offer a handy Screening Kit for AASTYs to test them all.

We recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022

Image Unavailable-77392-536

AASTY 11-45

Supplier: CUBE BIOTECH
Description: AASTYs (Acrylic acid-co-styrenes) - like AASTY 11-45 - are highly alternating copolymers, well-suited for the generation of native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 11-45 gets its name from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general lighter AASTYs, like 6-45 tend to be more aggressive, while heavier AASTYs, such as 11-45 show higher thermodynamic stability.

The exact composition of AASTY copolymers shows different extraction efficiency, depending on the lipid composition of the lipid bilayers being formulated into nanodiscs. As AASTY is made by controlled radical polymerization techniques, the dispersity of polymer molecular weight distribution is low, and the molecular weights are controlled. This means that excess AASTY copolymer can be removed by dialysis after nanodisc formation. Based on previous findings on SMA, it is the expectation that AASTY of different molecular weights will display different rates of nanodisc formation, extraction efficacy, and stability of resulting nanodiscs.

Every membrane protein solubilization needs to undergo a screening process before. The characteristic phospholipid environment surrounding the different membrane proteins in question performs differently well with each polymer. To support you in this process, we offer a handy Screening Kit for AASTYs to test them all. Additionally, we recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022

Image Unavailable-77392-648

HiYield Cell-free E. coli Lysate

Supplier: CUBE BIOTECH
Description: Our <i>E. coli</i> cell-free lysates are the isolated protein purification machinery of bacterial cells. They create a high-yielding and adjustable cell-free protein expression system.

Product Image-77393-308

PureCube Alprenolol Agarose

Supplier: CUBE BIOTECH
Description: PureCube Alprenolol resin provides highly-specific purification β-adrenergic receptors with maximum protein yield.

Product Image-77392-524

SMALP 140-I

Supplier: CUBE BIOTECH
Description: The SMALP products have been developed for biomedical purposes, in particular, for the solubilization of (biological) lipid membranes and isolation & purification of integral membrane proteins.
After solving the SMA + buffer powder in water, the product is ready-to-use. It can primarily used for all cell membrane hosts, including bacteria, yeast, and human cells. After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.

Product Image-77393-574

PureCube Fe-NTA MagBeads

Supplier: CUBE BIOTECH
Description: PureCube Fe-NTA MagBeads were developed to enrich phosphorylated proteins. They can also be used to purify His-tagged proteins as a secondary function.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
To process your orders without delay, please provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email CMD_NA@vwr.com or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:

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* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.

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The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at 1-800-932-5000.
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