You Searched For: Prosci

Catalog Number: (10748-958)

Supplier: Prosci

Description: Mitofusin 2 (MFN2) and the related protein MFN1 are mitochondrial membrane GTPase proteins that play a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. MFN2 is ubiquitously expressed, and found in both the ER and outer mitochondrial membrane. MFN2 has two key domains: a coiled coil region that mediates MFN2 binding and a GTPase domain that likely plays a role in fusion. Both domains are essential for embryonic development and may play a role in the pathobiology of obesity. Overexpression of MFN2 causes mitochondrial dysfunction and cell death.

Catalog Number: (10748-956)

Supplier: Prosci

Description: Mitofusin 1 (MFN1) and the related protein MFN2 are mitochondrial membrane GTPase proteins that play a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. MFN1 and MFN2 form homotypic and heterotypic complexes and coordinately regulate mitochondrial fusion and are essential for embryonic development. When ectopically expressed, MFN1 inhibits the apoptosis-associated amino-terminal conformation change in the apoptotic protein Bax but not its mitochondrial translocation, indicating that MFN1 is involved in the regulating the activation of Bax on the outer mitochondrial membrane.

Catalog Number: (10765-386)

Supplier: Prosci

Description: The 11B11 monoclonal antibody specifically reacts with mouse 14 kDa cytokine IL-4, expressed by the activated NKT cells and mast cells. IL-4 is species-specific and stimulates the proliferation and differentiation of B lymphocytes. It upregulates the expression of IgE receptors and class II MHC antigen.The 11B11 antibody neutralizes the IL-4 cytokine.

Catalog Number: (10765-452)

Supplier: Prosci

Description: The HI30 monoclonal antibody specifically reacts with the 180 kDa, 190 kDa, 205 kDa, and 220 kDa isoforms of the human leukocyte common antigen (LCA) CD45. It is expressed on lymphocytes, granulocytes, monocytes, thymocytes, and eosinophils, but not on mature erythrocytes, platelets, mature erythroid cells of bone marrow, and non-hematopoietic tissues. CD45 is essential for T cell activation and the tyrosine phosphatase activity of its intracellular region is integral for signal transduction. BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.

Catalog Number: (10765-466)

Supplier: Prosci

Description: Anti-PTPRC Rat Monoclonal Antibody (PE (Phycoerythrin)) [clone: RA3-6B2]

Catalog Number: (10765-382)

Supplier: Prosci

Description: The 11B11 monoclonal antibody specifically reacts with mouse 14 kDa cytokine IL-4, expressed by the activated NKT cells and mast cells. IL-4 is species-specific and stimulates the proliferation and differentiation of B lymphocytes. It upregulates the expression of IgE receptors and class II MHC antigen.The 11B11 antibody neutralizes the IL-4 cytokine.

Catalog Number: (10765-422)

Supplier: Prosci

Description: The RM4-5 monoclonal antibody specifically reacts with mouse CD4, also known as L3T4, a 55 kDa differentiation antigen expressed by the majority of thymocytes, subpopulations of mature T cells (like major histocompatibility complex class II-restricted T lymphocytes), a subset of natural killer T cells, and on pluripotent hematopoietic stem cells. CD4 binds to the major histocompatibility complex class II (MHC class II) and enhances T lymphocyte development and mature T cells functions. In T lymphocytes, CD4 binds to the cytoplasmic tail of enzyme tyrosine kinase (p56lck).Binding of RM4-5 is blocked by the anti-mouse CD4 clone GK1.5.

Catalog Number: (10765-454)

Supplier: Prosci

Description: The HI30 monoclonal antibody specifically reacts with the 180 kDa, 190 kDa, 205 kDa, and 220 kDa isoforms of the human leukocyte common antigen (LCA) CD45. It is expressed on lymphocytes, granulocytes, monocytes, thymocytes, and eosinophils, but not on mature erythrocytes, platelets, mature erythroid cells of bone marrow, and non-hematopoietic tissues. CD45 is essential for T cell activation and the tyrosine phosphatase activity of its intracellular region is integral for signal transduction. BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.

Catalog Number: (10766-350)

Supplier: Prosci

Description: The MEL-14 monoclonal antibody specifically reacts with L- selectin (CD62L), a receptor with lectin-like and Epidermal Growth Factor-like domains. The weight of the CD62L molecules depend on their origin, 74 kDa on lymphocytes or 95 kDa on neutrophils. In the mouse organism, CD62L can be expressed by most thymocytes, on subsets of B and T lymphocytes, monocytes, eosinophils, and neutrophils. The L-selectin binds sulfated, fucosylated, and glycosylated glycoproteins (MadCAM-1, GLYCAM-1, and CD 34). It mediates the migration of lymphocytes to the site of inflammation and their return to the peripheral lymphoid tissues and to the HEV (high endothelial venules). In vitro, L-selectin inhibits this binding and the lymphocyte extravasation into peripheral lymph nodes. After cell activation, it is quickly shed by neutrophils and lymphocytes.

Catalog Number: (10766-352)

Supplier: Prosci

Description: The MEL-14 monoclonal antibody specifically reacts with L- selectin (CD62L), a receptor with lectin-like and Epidermal Growth Factor-like domains. The weight of the CD62L molecules depend on their origin, 74 kDa on lymphocytes or 95 kDa on neutrophils. In the mouse organism, CD62L can be expressed by most thymocytes, on subsets of B and T lymphocytes, monocytes, eosinophils, and neutrophils. The L-selectin binds sulfated, fucosylated, and glycosylated glycoproteins (MadCAM-1, GLYCAM-1, and CD 34). It mediates the migration of lymphocytes to the site of inflammation and their return to the peripheral lymphoid tissues and to the HEV (high endothelial venules). In vitro, L-selectin inhibits this binding and the lymphocyte extravasation into peripheral lymph nodes. After cell activation, it is quickly shed by neutrophils and lymphocytes.

Catalog Number: (10766-360)

Supplier: Prosci

Description: The 17A2 monoclonal antibody specifically reacts with the mouse T lymphocytes receptor (TCR) associated CD3 complex, resulting in cellular activation and proliferation. CD3 is expressed by thymocytes and mature lymphocytes, and contains gamma, delta, and epsilon subunits, involved in the assembly, trafficking, and surface expression of T-cell receptor complex. The interaction between the T lymphocytes and the 17A2 antibody can be blocked by the anti-CD3e antibody, demonstrating that the 17A2 antibody recognizes the CD3 epsilon chain.

Catalog Number: (10766-294)

Supplier: Prosci

Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, the 20 kDa epsilon chain of the TCR complex. Together with the gamma and delta subunits of CD3, the epsilon subunits are involved in the assembly, trafficking, and surface expression of T-cell receptor complex. CD3 is expressed on thymocytes, mature T cells, and natural killer T cells, and the epsilon chain enhances the antigen recognition.The 145-2C11 antibody binds to the TCR complex and, depending on the conditions, initiates T cells activation, proliferation, and apoptosis. The soluble antibody seems to block lysis of target cells by antigen-specific cytotoxic T lymphocytes. The 145-2C11 antibody does not cross-react with the rat leukocytes, and it is used as a phenotypic marker for mouse T lymphocytes.

Catalog Number: (10766-306)

Supplier: Prosci

Description: The UCHT1 monoclonal antibody specifically reacts with the epsilon chain of the CD3/T lymphocyte antigen receptor complex. The CD3 complex contains gamma, delta, and epsilon chains, and it is part of the TCR complex, expressed by all mature T lymphocytes and by the thymocyte lineage. CD3 enhances the antigen recognition by signal transduction. Unlike HIT3a, another specific antibody of CD3, the UCHT1 antibody can stain both the surface and intracellular CD3 epsilon. The immobilized UCHT1 can cross-link with the TCR complex, enhancing cellular activation and proliferation.

Catalog Number: (10766-368)

Supplier: Prosci

Description: The GL-1 monoclonal antibody reacts specifically with the mouse B7-2 (also known as CD86), a costimulatory molecule expressed by B and T lymphocytes, macrophages (thioglycollate-induced from peritoneum), astrocytes and dendritic cells. The memory CD4+ T lymphocytes express CD86 (as mRNA and protein). CD86, and the B7-1 (CD 80) molecule, are ligands for CD152 and CD28, and influence the costimulatory interactions between lymphocytes B and T. B7-2 is also involved in the mouse natural killer cell-mediated cytotoxicity. The GL-1 antibody blocks the mixed lymphocyte reaction (MLR) and inhibits the T-cells stimulation by antigen-presenting cells. Mixtures of anti-B7-1 antibody and GL-1 were reported to inhibit the interaction between CD125 and its ligand with the in vivo priming of cytotoxic T lymphocytes.

Catalog Number: (10766-324)

Supplier: Prosci

Description: The MWReg30 monoclonal antibody specifically reacts with mouse CD41, a transmembrane glycoprotein also known as integrin alpha 2b GPIIb. It is expressed on hematopoietic progenitors, megakaryocytes, and platelets. It forms a receptor with CD61 (integrin beta 3) that binds adhesion molecules such as fibrinogen, fibronectin, von Willebrand factor, and thrombin. Defects or absence of CD41 has been found to lead to coagulation disorders. The expression profile of CD150+,CD48-, and CD41- can be used to identify hematopoietic stem cells.

Catalog Number: (10766-332)

Supplier: Prosci

Description: The VI-PL2 monoclonal antibody reacts with CD61, a type I integral transmembrane glycoprotein also known as integrin beta 3. CD61 forms a complex with CD41 (platelet gpIIb) and CD51 (integrin alphaV). It is expressed on osteoclasts, fibroblasts, platelets, macrophages, and megakaryocytes and appears to bind to fibronectin, vitronectin, thrombospondin, and fibrinogen and vWF. The VI-PL2 antibody is reported to be cross-reactive with baboon, rhesus, and cynomolgus.