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Supplier: STEMCELL Technologies

Description: Amine-labeling fluorescent dye for live/dead staining of mammalian cells - 100 tests.

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Supplier: STEMCELL Technologies

Description: Interleukin 33 (IL-33) is a pro-inflammatory cytokine from the IL-1 family. It binds to the ST2 receptor and activates NF-kB and MAPK pathways. IL-33 is expressed by epithelial cells, smooth muscle cells, and fibroblasts in various tissues and organs, as well as resting basophils, mast cells, eosinophils, natural helper cells, group 2 innate lymphoid cells, dendritic cells, and activated macrophages (Schmitz <i>et al.</i>; Yasuda <i>et al.</i>). It contributes to allergic inflammation by stimulating production of the cytokines IL-4, IL-5, and IL-13 in Th2 cells, and stimulates host defense against microbial and viral infections (Liew; Yasuda <i>et al.</i>). In the central nervous system, IL-33 is produced by endothelial cells and astrocytes. It induces proliferation of microglia and mediates production of pro-inflammatory cytokines (Yasuoka <i>et al.</i>).

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Description: Vascular endothelial growth factor (VEGF-165) is a heparin-binding homodimeric glycoprotein involved in embryonic vasculogenesis and angiogenesis. VEGF binds to VEGFR-1 (R1) and VEGFR-2 (R2), and activates Raf/MEK/ERK and PI3K/AKT pathways (Ferrara <i>et al.</i>). It plays an important role in neurogenesis both in vitro and in vivo (Storkebaum <i>et al.</i>). It has neurotrophic effects on neurons of the central nervous system and promotes growth and survival of dopaminergic neurons and astrocytes. VEGF also promotes growth and survival of vascular endothelial cells, monocyte chemotaxis, and colony formation by granulocyte-macrophage progenitor cells (Ferrara <i>et al.</i>).

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Description: Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of glycoproteins that regulate hematopoietic cell proliferation, differentiation, and function. It is a key cytokine involved in the production of neutrophils and the stimulation of granulocyte colony formation from hematopoietic progenitor cells (Metcalf and Nicola). G-CSF causes a range of effects including a transient reduction of SDF-1 expression (Petit <i>et al.</i>), the activation of metalloproteases that cleave VCAM-1 (Levesque <i>et al.</i>), and the release of norepinephrine from the sympathetic nervous system (Katayama <i>et al.</i>), leading to the release or mobilization of hematopoietic stem cells from the bone marrow into the periphery. The G-CSF receptor is expressed on a variety of hematopoietic cells, including myeloid-committed progenitor cells, neutrophils, granulocytes, and monocytes. In addition to hematopoietic cells, G-CSF is also expressed in cardiomyocytes, neuronal cells, mesothelial cells, and endothelial cells. Mouse G-CSF was first purified from cultures of the WEHI-3B myelomonocytic leukemia cell line as the inducer of the terminal differentiation of WEHI-3B and other myeloid leukemia cell lines (Nicola <i>et al.</i>). It was later cloned in monkey COS cells from a cDNA library prepared with mRNA derived from mouse fibrosarcoma NFSA cells that produce G-CSF constitutively (Tsuchiya <i>et al.</i>). Binding of G-CSF to its receptor leads to activation of the JAK/STAT, MAPK, PI3K, and AKT signal transduction pathways.

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Supplier: STEMCELL Technologies

Description: Granulocyte-macrophage colony-stimulating factor (GM-CSF) promotes the proliferation and differentiation of hematopoietic progenitor cells and the generation of neutrophils, eosinophils, and macrophages. In synergy with other cytokines such as stem cell factor, IL-3, erythropoietin, and thrombopoietin, it also stimulates erythroid and megakaryocyte progenitor cells (Barreda <i>et al.</i>). GM-CSF is produced by multiple cell types, including stromal cells, Paneth cells, macrophages, dendritic cells (DCs), endothelial cells, smooth muscle cells, fibroblasts, chondrocytes, and Th1 and Th17 T cells (Francisco-Cruz <i>et al.</i>). The receptor for GM-CSF (GM-CSFR) is composed of two subunits: the cytokine-specific α subunit (GMRα; CD116) and the common subunit βc (CD131) shared with IL-3 and IL-5 receptors (Broughton <i>et al.</i>). GM-CSFR is expressed on hematopoietic cells, including progenitor cells and immune cells, as well as non-hematopoietic cells. Recombinant human GM-CSF (rhGM-CSF) promotes the production of myeloid cells of the granulocytic (neutrophils, eosinophils and basophils) and monocytic lineages in vivo. It has been tested for mobilization of hematopoietic progenitor cells and for treating chemotherapy-induced neutropenia in patients. GM-CSF is able to stimulate the development of DCs that ingest, process, and present antigens to the immune system (Francisco-Cruz <i>et al.</i>).

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Supplier: STEMCELL Technologies

Description: Epidermal growth factor (EGF) is the founding member of the EGF family of proteins and is characterized by high affinity binding to various EGF receptors (EGFRs) and the production of mitogenic responses (Carpenter and Cohen). EGF promotes EGFR dimerization, resulting in activation of downstream pathways including PI3K, ERK1/2, JAK/STAT, β-catenin, and calcium signaling. EGF is secreted by the gut-associated salivary and Brunner’s glands, is found in a variety of body fluids, and stimulates cell proliferation and differentiation in rodent and neonatal human intestine (Wright <i>et al.</i>). EGF can stimulate the proliferation of mouse embryonic stem cells or induce the terminal differentiation/growth inhibition of A431 cells (Heo <i>et al.</i>).

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Supplier: STEMCELL Technologies

Description: Interleukin 22 (IL-22) is a class 2 α-helical cytokine that signals through the class 2 cytokine receptor and activates the JAK/STAT pathway. IL-22 is secreted by Th1, Th2, Tc22, and γδ T cells, dendritic, mast and NK cells. It stimulates expression of antimicrobial peptides from epithelial cells, thus hindering bacterial infections. Depending on the interactions with other cytokines, IL-22 can either promote inflammation or prevent tissue destruction by regulating host defense and tissue homeostasis at barrier surfaces (Sonnenberg <i>et al.</i>).

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Description: Immunomagnetic positive selection of any cells with your own mouse IgG1 antibody.

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Description: Column-free immunomagnetic positive selection of human CD8+ cells for HLA analysis.

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Description: Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD2, PE-conjugated.

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Description: Mouse monoclonal IgG1 antibody against human, chimpanzee CD45, APC-conjugated.

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Description: Immunomagnetic positive selection of particle-free human CD3+ cells.

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Description: Ciliary neurotrophic factor (CNTF) is a neurotrophic factor that belongs to the four-helix bundle cytokine family and is structurally related to interleukin 6 (IL-6), interleukin 11 (IL-11), leukemia inhibitory factor (LIF), and oncostatin M (OSM). CNTF binds to its receptor CNFTRα and induces formation of a heterodimer of the signal transducing IL-6 receptor gp130 and LIF receptor (LIFR)-β, which triggers JAK/STAT, ERK, and PI3K signaling cascades (Schuster <i>et al.</i>). CNTF plays an important role in neurogenesis and the differentiation of neural stem cells and has been suggested to possess a therapeutic role in treating neurological disorders (Ding <i>et al.</i>; Oppenheim <i>et al.</i>). CNTF has also been shown to protect rod photoreceptors from light-induced damage and have therapeutic effects on retinal degenerative diseases caused by genetic defect or damage induced by toxins, autoantibodies, or strong light (Pernet <i>et al.</i>; Rhee <i>et al.</i>). Another therapeutic role of CNTF has been reported in protecting oligodendrocytes from death induced by apoptosis (Louis <i>et al.</i>). Additionally, CNTF is commonly used to differentiate human pluripotent stem cell (hPSC)-derived neural progenitor cells into astrocytes (Krencik and Zhang). This product is animal component-free.

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Catalog Number: (MSPP-100-0934)

Supplier: STEMCELL Technologies

Description: R-Spondin-3 is a member of thrombospondin type 1 repeat (TSR-1) superfamily that is involved in the canonical Wnt/β-catenin signaling pathway (de Lau <i>et al.</i>). R-spondin proteins are characterized by two furin-like repeats at the amino terminus and thrombospondin domain located near the carboxyl terminus (de Lau <i>et al.</i>). R-spondin-3 expression is associated with ovarian cancer (Gu <i>et al.</i>), prostate cancer (Mesci <i>et al.</i>), and differentiation of intestinal epithelial cells in diabetes mellitus (Shan <i>et al.</i>). In a transgenic mice model, the expression of R-Spondin-3 induces the expansion of Lgr5+ stem cells, Paneth cells, and Lgr4+ cells, promoting the intestinal stem cell compartment (Hilkens <i>et al.</i>). This protein contains a His-residue tag at the carboxyl end of the polypeptide chain.

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Catalog Number: (MSPP-100-1083)

Supplier: STEMCELL Technologies

Description: Goat polyclonal antibody against rabbit IgG (H+L).

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Description: Immunodensity depletion of granulocytes for HLA analysis.

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