You Searched For: 0000013509

Catalog Number: (PI28363)

Supplier: Invitrogen

Description: These space-saving stock solutions are ideal for quick preparation of standard Tris-glycine (pH 8.5) transfer buffer which is used for Western blotting.

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Catalog Number: (PI22308)

Supplier: Invitrogen

Description: Thermo Scientific Pierce EMCS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length spacer arm (9.4 angstroms).

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Supplier: Invitrogen

Description: Thermo Scientific Pierce DSP (Lomant's Reagent) is a water-insoluble, homo-bifunctional N-hydroxysuccimide ester (NHS ester) crosslinker that is thiol-cleavable, primary amine-reactive, and useful for many applications. DSP contains an amine-reactive NHS ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7–9 to form stable amide bonds and releasing N-hydroxy-succinimide. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS ester crosslinking reagents. DSP is non-sulfonated and insoluble in water, so it must first be dissolved in an organic solvent and then added to the aqueous reaction mixture. Because DSP does not possess a charged group, it is lipophilic and membrane-permeable and so useful for intracellular and intramembrane conjugation. A sulfonated analog of DSP (DTTSP) is water soluble. DSS, the non-cleavable analog of the DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.

SDS Certificates

Catalog Number: (PI22317)

Supplier: Invitrogen

Description: Thermo Scientific Pierce Sulfo-SMPB is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length aromatic spacer arm (11.6 angstroms).

SDS

Catalog Number: (PI22615)

Supplier: Invitrogen

Description: Thermo Scientific Pierce L-Photo-Methionine is an analog of L-methionine that can be incorporated into proteins during synthesis and then light-activated to covalently crosslink interacting proteins in cells.

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Catalog Number: (PI22331)

Supplier: Invitrogen

Description: Thermo Scientific Pierce BMB is a mid-length, maleimide crosslinker for covalent, irreversible conjugation between sulfhydryl groups (e.g., protein or peptide cysteines).

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Supplier: Invitrogen

Description: Blocker BSA in PBS or TBS are ready-to-use, 1X PBS or TBS solutions of 10X (10%) bovine serum albumin protein for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.

SDS Certificates

Supplier: Invitrogen

Description: This luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate for Western blotting provides very stable light output especially for documentation with CCD camera imagers with sensitivity in the mid-femto range.

SDS Certificates

Catalog Number: (PI20308)

Supplier: Invitrogen

Description: SDS-Out™ SDS Precipitation Kit provides convenient removal of SDS from small-volume (0.1 to 10 mL) protein samples.

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Supplier: Invitrogen

Description: Thermo Scientific Guardian Peroxidase Conjugate Stabilizer/Diluent preserves the functional integrity and activity of horseradish peroxidase conjugated antibodies and other proteins at very dilute concentrations for long-term storage.

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Catalog Number: (PI88438)

Supplier: Invitrogen

Description: Thermo Scientific Heavy and Light Amino Acids are used to specifically analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) quantification kits.

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Catalog Number: (PI24612)

Supplier: Invitrogen

Description: The Pierce Silver Stain Kit is a rapid, ultra-sensitive, and versatile silver stain system for protein detection in polyacrylamide gels, yielding consistent and reliable results. It is a metallic silver (Ag) protein stain that yields a remarkably clear and uniform gel background. In standard mini gels, proteins are detectable at greater than 0.25 ng per band or spot. The protocol has been optimized for flexibility by allowing short or overnight gel fixation and staining steps without affecting staining performance (sensitivity or clarity). A short (one-minute) sensitization step, performed after gel fixation, yields results that are free of the characteristically dark or blotchy backgrounds often seen with homemade or other commercially available silver stains. This feature is beneficial when performing densitometric analysis of silver stained gels.

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Catalog Number: (PI25108)

Supplier: Invitrogen

Description: Thermo Scientific Pierce Triethylamine (TEA) is a high-purity solvent that is used in certain HPLC protocols, such as weak anion exchange, to resolve certain tryptic peptides on a reverse-phase column.

SDS Certificates

Catalog Number: (PI25003)

Supplier: Invitrogen

Description: Thermo Scientific Pierce Heptafluorobutyric Acid (HFBA) is manufactured and tested to meet strict specifications that ensure superior performance for use as an ion-pairing agent.

SDS Certificates

Catalog Number: (PI21217)

Supplier: Invitrogen

Description: Thermo Scientific EZ-Link Sulfo-NHS-Biotin is a short-chain, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

SDS Certificates

Catalog Number: (PI21277)

Supplier: Invitrogen

Description: The Pierce™ His Protein Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with His-tagged fusion proteins.

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