You Searched For: SOUTHERN BIOTECHNOLOGY MS

Catalog Number: (100243-896)

Supplier: Southern Biotechnology

Description: The monoclonal antibody 2F1 reacts with mouse killer cell lectin-like receptor G1 (KLRG1; formerly known as mouse MAFA), a homodimeric member of the lectin-like type 2 transmembrane receptor family that contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains. These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1. The 2F1 antibody stains 30-60% of NK1.1+CD3- splenocytes, and a small fraction of T cells in all mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL). Cell surface expression of KLRG1 is up-regulated by expression of MHC class I molecules. The effect of MHC class I expression is indirect, and can be mediated by interactions with class I-specific Ly49 inhibitory receptors.

Catalog Number: (100243-682)

Supplier: Southern Biotechnology

Description: CD90 (Thy-1), a GPI-anchored molecule and one of the smallest members of the immunoglobulin superfamily of cell surface receptors, consists of a single V-set domain. It is expressed on thymocytes, peripheral T lymphocytes, some intraepithelial T lymphocytes, and neurons of all mouse strains. The monoclonal antibody G7 stimulates T-cell proliferation and IL-2 secretion via signaling through the T-cell receptor/CD3 complex. G7 has also been reported to promote apoptosis of thymocytes and CTL clones and to mediate adhesion of thymocytes to thymic stroma.

Catalog Number: (100243-300)

Supplier: Southern Biotechnology

Description: CD22, also known as Lyb-8, is a heterodimeric transmembrane glycoprotein and a member of a structurally related group of immunoglobulin (Ig) superfamily domain-containing proteins called the sialoadhesin family. It is detected in the cytoplasm early in B cell development (late pro-B cell stage), appears on the cell surface concomitant with IgD, and is found on most IgM+IgD+ mature B lymphocytes. Expression is lost with terminal differentiation of B cells and is absent on plasma cells. Activation of B cells via cross-linking of surface Ig increases CD22 expression. CD22 associates with the B cell receptor (BCR) complex and mediates intercellular adhesion. Its intracellular domain is phosphorylated after antigen receptor cross-linking and is involved in negative regulation of B cell activation.

Catalog Number: (100243-938)

Supplier: Southern Biotechnology

Description: CD45RC represents an exon C-dependent isoform of CD45 glycoprotein. It is expressed at high density on all B lymphocytes, at intermediate density on most CD8+ splenic T cells, and on two subpopulations of CD4+ splenic T cells with high and low density of expression. CD45RC is also present on a small subset of thymocytes.

Supplier: Southern Biotechnology

Description: CD45 is the common leukocyte antigen and is expressed on all cells of hematopoietic origin except erythrocytes. CD45 exists as a number of different isoforms which result from alternative RNA splicing of exons 4, 5 and 6. The I3/2.3 monoclonal antibody recognizes a framework epitope present on all CD45 isoforms. The CD45 molecule has been implicated as playing a key role in thymic development and in antigen-driven proliferation of T cells.

Catalog Number: (100244-098)

Supplier: Southern Biotechnology

Description: The “classical” MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic α chains noncovalently associated with the invariant β2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny. The monoclonal antibody 34-1-2S binds to a common determinant in the α3 domains of H-2Kd and H-2Dd in the presence or absence of β2 microglobulin. It cross reacts with the α3 domain of H-2Kb.

Catalog Number: (100246-176)

Supplier: Southern Biotechnology

Description: CD14 is a 55 kDa glycosphosphatidylinositol (GPI)-anchored single chain glycoprotein. It is expressed on monocytes, macrophages, dendritic cells, and Langerhans cells. It is also weakly expressed on neutrophils. CD14 appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.

Catalog Number: (100245-822)

Supplier: Southern Biotechnology

Description: FITC Anti-Human IgG3 Hinge antibody for use in flow cytometry, immunohistochemistry, and western blotting assays.

Catalog Number: (100245-578)

Supplier: Southern Biotechnology

Description: Chicken Bu-1b, a product of the Bu-1b allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes but not on granulocytes, erythrocytes, or thrombocytes. In chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion. The monoclonal antibody 5-11G2 reacts with lymphoid cells from CHA and H.B14Ab strains but does not react with cells from H.B14Aa

Catalog Number: (100245-556)

Supplier: Southern Biotechnology

Description: Like their mammalian counterparts, avian MHC Class I molecules, also known as B-F antigens, consist of a highly polymorphic α-chain noncovalently bound to the invariant β2-microglobulin subunit. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes which are usually cytotoxic T cells. The monoclonal antibody F21-21 also reacts with turkey β2-microglobulin.

Catalog Number: (100242-358)

Supplier: Southern Biotechnology

Description: Purified Anti-Human G-CSF antibody for use in immunohistochemistry / immunocytochemistry, ELISA, and drug delivery assays.

Catalog Number: (100241-892)

Supplier: Southern Biotechnology

Description: Purified Mouse IgG1 for use as a control in flow cytometry, immunohistochemistry / immunocytochemistry, western blotting, multiplex, and in vitro assays and as a standard in ELISA.

Catalog Number: (100242-040)

Supplier: Southern Biotechnology

Description: Rabbit IgG-FITC for use as a control in flow cytometry and immunohistochemistry / immunocytochemistry assays.

Catalog Number: (100242-030)

Supplier: Southern Biotechnology

Description: Goat F(ab’)2 IgG-FITC for use as a control in flow cytometry and immunocytochemistry assays.

Catalog Number: (100242-366)

Supplier: Southern Biotechnology

Description: Goat Anti-Mouse IgM-TRITC antibody with minimal reactivity to human proteins for use in flow cytometry and immunohistochemistry / immunocytochemistry assays.

Supplier: Southern Biotechnology

Description: Goat Anti-Mouse IgG2a-PE antibody with minimal reactivity to human proteins for use in flow cytometry assays.