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MMP Colorimetric Substrate I

Supplier: Anaspec Inc
Description: Matrix Metalloproteinases (MMPs) are a large family of endopeptidases. Collectively, MMPs can degrade all kinds of extracellular matrix proteins, and can also process a number of bioactive molecules. They are known to be involved in the cleavage of cell surface receptors, the release of apoptotic ligands, and chemokine/cytokine inactivation. MMPs are also thought to play a major role in cell behaviors such as cell proliferation, migration (adhesion/dispersion), differentiation, angiogenesis, apoptosis, and host defense.
This peptide is an exceptional MMP-2 (gelatinase A) and MMP-9 (gelatinase B) substrate: this peptide is used for the continuous spectrophotometric assay of MMP-2 and MMP-9 in combination with a color-developing thiol-reactive agent, 4,4’-dithiodipyridine or Ellman’s Reagent (as indicators). This thiol peptide-enzyme reaction has a Km of 0.004 M and a kcat of 103 s-1. Using this substrate, collagenase can be detected at concentrations as low as 2 ng/mL. HPLC and tandem mass spectrometry are also used to analyze MMP-2 and MMP-9 in conjunction with this peptide substrate.
Sequence:Ac-PLG-SCH[CH2CH(CH3)2]-CO-LG-OC2H5
MW:655.9 Da
% peak area by HPLC:95
Storage condition:-20° C

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Chloroform 99,8 ACS

Supplier: MP Biomedicals
Description: Chloroform is typically used as a solvent and as a cleansing agent. Procedures have been described for the use of chloroform in lambda plaques storage, lambda cDNA storage, the removal of mineral oil from PCR reaction samples, oligonucleotide purification, a hydroxyl radical footprinting protocol, a transcriptional run-on assay protocol, and an overlay assay for beta-galactosidase activity. It has also been used with phenol in such procedures as DNA recovery from polyacrylamide gels, ethidium bromide removal from DNA preparations, lysis protocols for plasmid DNA isolation, RNase removal, and purification of yeast DNA. A protocol describes the use of chloroform in a high-performance thin-layer chromatography protocol for sphingomyelin analysis. Chloroform can also be used in chloroform/methanol mixtures for the isolation of cardiolipids from Geobacillus stearothermophilus and their subsequent MS analysis. The isolation of the bacteriocin amylovorin L471 from Lactobacillus amylovorus DCE 471 in culture broth has been reported, using chloroform/methanol extraction and precipitation in the procedure. Chloroform/2-butanol mixtures can be used for the extraction of steroid sulfates for analysis by nanoelectrospray ionization mass spectrometry.

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L-Arginine hydrochloride for SILAC

Catalog Number: (PI89989)
Supplier: Invitrogen
Description: Heavy and Light Amino Acids are used to specifically analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) quantification kits. Store at 2 - 8˚C.

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Chloroform 99 Stabilised Molecular_Biology

Supplier: MP Biomedicals
Description: Chloroform is a common laboratory reagent favored for use as a solvent due to the compounds relative unreactivity and miscibility with most organic compounds. Chloroform is a colorless, volatile chlorinated organic solvent whose vapors have a narcotic effect.
Chloroform is use as a solvent for fats, oils, rubber, alkaloids, waxes, gutta-percha, resins; as cleansing agent; in fire extinguishers to lower the freezing temperature of carbon tetrachloride. Procedures have been described for the use of chloroform in lambda plaques storage, lambda cDNA storage, the removal of mineral oil from PCR reaction samples, oligonucleotide purification, a hydroxyl radical footprinting protocol, a transcriptional run-on assay protocol, and an overlay assay for beta-galactosidase activity. It has also been used with phenol in such procedures as DNA recovery from polyacrylamide gels, ethidium bromide removal from DNA preparations, lysis protocols for plasmid DNA isolation, RNase removal, and purification of yeast DNA. A protocol describes the use of chloroform in a high-performance thin-layer chromatography protocol for sphingomyelin analysis. Chloroform can also be used in chloroform/methanol mixtures for the isolation of cardiolipids from Geobacillus stearothermophilus and their subsequent MS analysis. The isolation of the bacteriocin amylovorin L471 from Lactobacillus amylovorus DCE 471 in culture broth has been reported, using chloroform/methanol extraction and precipitation in the procedure. Chloroform/2-butanol mixtures can be used for the extraction of steroid sulfates for analysis by nanoelectrospray ionization mass spectrometry.
Physical Appearance: Liquid
Vapor Density: 4.36 (air = 1) (Lit.)
Vapor Pressure: 211 hPa at 20 °C(Lit.)
Refractive Index: n20/D 1.445(Lit.)
Solubility: Soluble in water (1 mL/200 mL water); miscible with ethanol, benzene, ether, petroleum ether, carbon tetrachloride, carbon disulfide, oils.

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Image Unavailable-PI88427

L-Arginine hydrochloride, lyophilized powder for SILAC

Catalog Number: (PI88427)
Supplier: Invitrogen
Description: Thermo Scientific Heavy and Light Amino Acids are used to specifically analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) quantification kits.

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Ammonium acetate 97, crystals ACS suitable for use as excipient

Supplier: Spectrum Chemicals
Description: Ammonium Acetate, Crystal, Reagent, ACS is a chemical compound that comes as a crystalline white solid. Its uses vary from biodegradable de-icer as well as being used in dialysis as part of a protein purification stage that is useful in removing contaminant via diffusion. It is also used in an aqueous solution as a buffer for ESI mass spectrometry of proteins. As an ACS grade reagent, Spectrum Chemical manufactured compound is used as the quality standard against which other substances are grade and has met the toughest regulatory standards for quality and pureness

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Image Unavailable-AAJ67635-EXK

ɑ-Cyano-4-hydroxycinnamic acid, ultrapure MALDI Matrix

Catalog Number: (AAJ67635-EXK)
Supplier: Thermo Scientific Chemicals
Description: Applications: For use as a matrix in MALDI mass spectrometry analysis

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Caffeic acid 99 for synthesis

Supplier: Spectrum Chemicals
Description: Caffeic Acid, also known as trans-Caffeate, is a key intermediate in the biosynthesis of lignin and is also used as a matrix in MALDI mass spectrometry.

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1,1,1,3,3,3-Hexafluoro-2-propanol ≥99%, clear liquid

Supplier: MP Biomedicals
Description: 1,1,1,3,3,3-Hexafluoro-2-propanol is polar and exhibits strong hydrogen bonding properties enabling it to dissolve substances that serve as hydrogen-bond acceptors. 1,1,1,3,3,3-Hexafluoro-2-propanol is a polar solvent of high ionizing power and facilitates Friedel–Crafts-type reactions, using covalent reagents in the absence of a Lewis acid catalys. 1,1,1,3,3,3-Hexafluoro-2-propanol clusters catalyzes the epoxidation of cyclooctene and 1-octene with hydrogen peroxide.
It is a speciality solvent effective for solubilizing a wide range of polymers, including those that are not soluble in the most common organic solvents: such as polyamides, polyacrylonitriles, polyacetals, polyesters (e.g. polyglycolide), and polyketones. It has also found use in biochemistry to solubilize peptides and to monomerize β-sheet protein aggregates. It can be used as an acid in volatile buffers for ion pair HPLC - mass spectrometry of nucleic acids. 1,1,1,3,3,3-Hexafluoro-2-propanol was used for preparing hexafluoroalcohol-functionalized methacrylate polymers for lithographic/nanopatterning materials.

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Imperial™ Protein Stain, Thermo Scientific

Supplier: Invitrogen
Description: Imperial Protein Stain is a ready-to-use colorimetric stain formulated with coomassie dye R-250 that delivers consistent nanogram-level detection of proteins in polyacrylamide electrophoresis gels or nitrocellulose membranes.

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TraceSELECT™ Fluka™ Cesium Chloride - Lanthanum Chloride Buffer, Honeywell Reseach Chemicals

Catalog Number: (BJ95164-250ML)
Supplier: Honeywell Research Chemicals
Description: TraceSELECT™, for flame atomic absorption spectrometry.

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Image Unavailable-EM1.02037.1000

Cesium Chloride-Aluminium Nitrate Buffer Solution acc. to Schuhknecht and Schinkel for the Determination of Sodium, Potassium and Lithium by Flame Spectrometry, MilliporeSigma

Catalog Number: (EM1.02037.1000)
Supplier: MilliporeSigma
Description: Accordingto Schuhknecht And Schinkel For The Determination Of Sodium, Potassium And Lithium By Flame Spectrometry

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2-(1-Naphthyl)-5-phenyloxazole 99 laser, scintillation_for

Supplier: Thermo Scientific Chemicals
Description: 2-(1-Naphthyl)-5-phenyloxazole 99 laser, scintillation_for laser grade and suitable for scintillation spectrometry

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Product Image-470328-144

Glass Equilateral Prisms

Supplier: Wards
Description: Designed for educational purposes and science classroom demonstrations in optics, light refraction, spectrometry, and wavelengths.

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2-(1-Naphthyl)-5-phenyloxazole 99,0

Catalog Number: (TCN0068-001G)
Supplier: TCI America
Description: [for scintillation spectrometry] CAS Number: 846-63-9 MDL Number: MFCD00019714 Molecular Formula: C19H13NO Molecular Weight: 271.32 Purity/Analysis Method: <gt/>99.0% (HPLC,N) Form: Crystal Melting point (°C): 106

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2,5-Diphenyloxazole 99,0

Supplier: TCI America
Description: [for scintillation spectrometry] CAS Number: 92-71-7 MDL Number: MFCD00005306 Molecular Formula: C15H11NO Molecular Weight: 221.26 Purity/Analysis Method: <gt/>99.0% (HPLC,N) Form: Crystal Melting point (°C): 72

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