HEPES-2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulfonic acid, powder acid-free, Corning®

Supplier: Corning

Synonyms: 4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acidN-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulphonic acidN-2-Hydroxyethylpiperazine-N′-2-ethanesulfonic acid4-(2-Hydroxyethyl)piperazin-1-ylethanesulfonic acidN-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulphonic acid)N-2-Hydroxyethylpiperazine-N′-2-ethanesulphonic acidHEPES free acid

61-034-RM 61-034-RR 61-034-RO 61-034-RO 61-034-RR 61-034-RM
45000-694EA 275.65 USD
45000-694 CA45000-692 45000-692 CA45000-696 45000-696 CA45000-694
HEPES-2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulfonic acid, powder acid-free, Corning®
HEPES (4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid)

HEPES (N-2-hydroxyethylpiperazine-N’-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2


Unlike the low pKa of bicarbonate, HEPES has a pKa of 7.66 making it a much stronger buffer in the pH 7.2-7.6 range than bicarbonate buffer. Potential disadvantages of HEPES include toxicity over 100 mM and lack of nutritive value to the cell.

Formula: C₈H₁₈N₂O₄S
MW: 238.31 g/mol
Storage Temperature: Ambient
MDL Number: MFCD00006158
CAS Number: 7365-45-9
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