Protein identification is based on both antibody reactions and antigens in this Western Blot activity.
- Materials for Six Blots Included
- Instructions with Background Information
- Students Research Protein Identification
A denaturing SDS polyacrylamide gel separates the proteins, which are transferred or blotted to a nylon membrane. The membrane is then exposed sequentially to solutions containing the primary antibody, followed by a secondary antibody that is coupled to an enzyme.
The membrane is soaked in a substrate solution to develop the color reaction, which results in identifying the antigen as a band, and the molecular weights of the visible bands are measured using pre-stained protein markers.
Ordering information: This kit contains negative lyophilized control BSA, high and low concentration (lyophilized); an anti-BSA protein antibody; secondary antibody conjugate; hydrogen peroxide; peroxide co-substrate, 10X; blocking buffer; PBS buffer; pre-stained protein standard marker (lyophilized); tris-glycine-SDS electrophoresis buffer; powdered milk; and instructions with background information. The kit requires but does not include three polyacrylamide gels, MV-10 vertical gel electrophoresis apparatus, DC power supply, automatic micropipet, fine micropipet tips, glacial acetic acid, methanol, and distilled or deionized water.
Delivery information: This kit is shipped ambient. Store lyophilized proteins at room temperature.
