Concanavalin A Agarose for Glycoprotein Purification, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' Concanavalin A (Con A) Agarose is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues
G-Biosciences' Con A Agarose consists of Con A coupled to 4% agarose by the cyanogen bromide method. Con A is a tetrameric metalloprotein lectin isolated from Canavalia ensiformis (jack bean). To maintain its binding characteristics the presenceof both Mn²⁺ and Ca²⁺ is essential. Each subunit of Con A utilizes one calcium and one manganese ion, and these cations can be removed under acidic conditions, abolishing the carbohydrate-binding activity.
G-Biosciences' Con A Agarose can also be used in other application areas including purification of enzyme-antibody conjugates, purification of IgM and separation of membrane vesicles. The ligand density of Con A Agarose is 10-16 mg Con A per mL resin, and it's binding capacity is 20-50 mg thyroglobulin per mLresin.
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