Protein Extraction and Lysis Buffer (PE LB™) Systems, G-Biosciences

Supplier: G-Biosciences
786-181T 786-179 786-191 786-411 786-177 786-180 786-187 786-185 786-176 786-178 786-186 786-189 786-411 786-181 786-177 786-188 786-176 786-179 786-181T 786-180 786-181 786-178
76286-160EA 448.26 USD
76286-160 82021-546 82023-266 95057-584 CA82023-266 CA95057-584 76286-158 82022-592 CA95057-704 95057-704 CA82021-546 76286-162 CA82022-598 CA82022-596 76286-164 82022-598 76286-166 CA82022-594 82022-596 76286-156 82022-594 CA82022-592
Protein Extraction and Lysis Buffer (PE LB™) Systems, G-Biosciences
Buffers Extraction Buffers
Lysis and extraction of biologically active proteins from cellular and tissue samples is the first critical step for biochemical analysis

The Protein Extraction & Lysis Buffer (PE LB™) systems ensure good protein recovery, while maintaining the biological activity of the proteins. The solubilized proteins are suitable for enzyme assays (see figure), electrophoresis, folding studies, chromatographic studies and many other downstream applications.

The PE LB™ systems offer a wide selection of buffers for lysis and extraction of proteins from bacteria, yeast, animal cells and tissues. The PE LB™ systems are based on a proprietary combination of organic buffering agents, mild non-ionic detergents, and a combination of various salts to enhance extraction of proteins and maintain stability of biological activities of the proteins.

Depending on the application, additional agents such as chelating agents, reducing agents and protease and phosphatase inhibitors may be added to the PE LB™ buffer system.

The PE LB™ systems are compatible with most downstream applications including enzyme assays, ELISA, chromatographic applications, gel electrophoresis, western blotting and protein folding procedures.

The correct selection of lysis and extraction buffers requires knowledge of the proteins of interest and the stability of their biological activities
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