Phenol : chloroform : iso-amyl alcohol (25:24:1) (v/v/v) ≥99%, Biotechnology Grade, low pH, VWR®
Supplier: VWR
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Danger
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Synonyms:
Carbolic acid, Hydroxybenzene, Phenic acid
Phenol / Chloroform #2- A 25:24:1 mixture of phenol, chloroform, and isoamyl alcohol useful for preparing RNA with maximal recovery of poly (A+) RNA for subsequent mRNA purification and generation of cDNA.
- Manufactured Using Exceptionally High Purity Phenol that is Double Distilled & Packaged Under Nitrogen
- Liquid Phenols Offer a Safer, Easier Alternative to Crystalline Phenol
- Optimized pH for the Purification of RNA
- Long Term Stability > One Year
For RNA purification, it is best to use a phenol or phenol:chloroform mixture with a pH of 4.5 to 5.5. Although phenol alone will purify RNA in these extractions, messenger RNA can collect with the DNA at the inter-phase in the complete absence of chloroform. The ratio of phenol to chloroform can vary from no chloroform to a 1:1 mixture, depending on the individual tissue and the character of the RNA being purified. For many applications, a 5:1 ratio of phenol:chloroform is a recommended starting point. The phenol used for extraction protocols must be highly purified and essentially free of contamination by phenolic acid, a product of phenol oxidation. VWR’s phenol products are manufactured from the purest crystalline phenol to ensure optimal results.
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MDL Number:
MFCD00133763 CAS Number: 136112-00-0 UN: 2810 ADR: 6.1,II |
Specification Test Results
| O.D.@ 330 nm | ≤0.2 |
| O.D.@ 405 nm | ≤0.1 |
| O.D.@ 510 nm | ≤0.1 |
| pH @ 25 °C | 4.9 – 5.5 |
| Purity | ≥99 % |
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