Phenol : chloroform : iso-amyl alcohol (125:24:1) (v/v/v) ≥99.0%, Biotechnology Grade, VWR®
Supplier: VWR
|
Danger
|
Synonyms:
Carbolic acid, Hydroxybenzene, Phenic acid
Phenol Chloroform 5:1 Solution. An optimised mixture that can be used directly to replace the phenol and chloroform recommended in the one-step phenol purification method.
- Manufactured using exceptionally high purity phenol that is double distilled & packaged under nitrogen
- Liquid phenols offer a safer, easier alternative to crystalline phenol
- Optimized pH for the Purification of RNA
- Long term stability > one year
For RNA purification, it is best to use a phenol or phenol:chloroform mixture with a pH of 4.5 to 5.5. Although phenol alone will purify RNA in these extractions, messenger RNA can collect with the DNA at the inter-phase in the complete absence of chloroform. The ratio of phenol to chloroform can vary from no chloroform to a 1:1 mixture, depending on the individual tissue and the character of the RNA being purified. For many applications, a 5:1 ratio of phenol:chloroform is a recommended starting point. The phenol used for extraction protocols must be highly purified and essentially free of contamination by phenolic acid, a product of phenol oxidation. VWR’s phenol products are manufactured from the purest crystalline phenol to ensure optimal results.
|
MDL Number:
MFCD00133763 CAS Number: 136112-00-0 UN: 2810 ADR: 6.1,III |
Specification Test Results
| Abs.@ 330 nm | 0.2 |
| Abs.@ 405 nm | 0.1 |
| Abs.@ 510 nm | 0.1 |
| pH @ 25 °C | 4.3 – 4.7 |
| Purity | 99.0 |
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...